产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Endothelial Nitric Oxide Synthase (Ser-632), phospho-specific
目录 :
MBS470012
规格 :
0.1毫升
价格 :
380美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
产品信息
目录号 :
MBS470012
产品类型 :
抗体
产品全称 :
Endothelial Nitric Oxide Synthase (Ser-632), phospho-specific
产品简称 :
Endothelial Nitric Oxide Synthase (Ser-632)
产品名称同义词 :
endothelial Nitric Oxide Synthase; eNOS; ecNOS; NOS-III
其他名称 :
endothelial nitric oxide synthase; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; OTTHUMP00000212826; OTTHUMP00000213183; OTTHUMP00000213184; OTTHUMP00000213185; nitric oxide synthase 3 (endothelial cell); Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III
其他基因名称 :
内皮细胞一氧化氮合酶;内皮细胞一氧化氮合酶;eNOS;ECNOS
UniProt数据库进入名 :
NOS3_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG1
宿主 :
小鼠
反应物种 :
人类, 大鼠, 小鼠
特异性 :
The antibody detects a 140 and 120 kDa* bands on SDS-PAGE immunoblots of human umbilical vein endothelial cells, but these bands are not observed after lambda phosphatase treatment. The 120 kDa band may be a truncated form of eNOS.
纯度 :
蛋白质A纯化
形式 :
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
储存稳定性 :
Store at -20 degree C. Do not aliquot. Stable for 1 year.
检测过的应用 :
酶联免疫吸附测定, 免疫印迹
应用笔记 :
ELISA: 1:2000. Western Blot: 1:500
其它信息1 :
Immunogen: Phospho-eNOS (Ser-632) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-632 in mouse eNOS. This sequence is conserved in human (Ser-633) and rat (Ser-632) eNOS, and has low homology to other NOS family members.
产品描述 :
Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca(2+) flux and Ser-1177 phosphorylation.
产品引用 :
Xie, Q.W. et al. (1992) Science 256:225. Musicki, B. et al. (2005) Proc. Natl. Acad. Sci.102(33):11870. Mount, P.F. et al. (2007) J Mo.l Cell. Cardiol. 42(2):271.
NCBI GI登录号 :
825652
UniProt数据库登录号 :
P29474
NCBI分子量 :
133,289 Da
NCBI信号通路 :
ACE Inhibitor Pathway 198763!!Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Formation Of Platelet Plug Pathway 106029!!Hemostasis Pathway 106028!!Metabolic Pathways 132956!!Metabolism Of Nitric Oxide Pathway 106222
NCBI总结 :
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt数据库总结 :
Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Catalytic activity: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+. Cofactor: Heme group.Binds 1 FAD.Binds 1 FMN.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Ref.19 Ref.20. Subunit structure: Homodimer. Interacts with NOSIP and NOSTRIN. Ref.19 Ref.20. Subcellular location: Cell membrane. Membrane caveola. Cytoplasm cytoskeleton. Golgi apparatus. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Ref.19 Ref.20 Ref.21 Ref.22. Tissue specificity: Platelets, placenta, liver and kidney. Ref.18. Polymorphism: Variation in NOS3 seem to be associated with susceptibility to coronary spasm. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.
规格 :
0.1毫升
价格 :
380美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。