抗体

FAK (Tyr-397), phospho-specific, Mouse Monoclonal IgG1

MBS470013

0.1毫升

380美元

单克隆

小鼠

未共轭

磷酸化

免疫印迹, 酶联免疫吸附测定

抗体

FAK (Tyr-397), phospho-specific, Mouse Monoclonal IgG1

FAK (Tyr-397)

粘着激酶1;pp125FAK

单克隆

IgG1

小鼠

人类, 大鼠, 小鼠, 兔

The antibody detects a 125 kDa* protein on SDS-PAGE immunoblots of untreated HUVEC cells. This phosphorylated band is greatly reduced after treatment with alkaline phosphatase.

蛋白质A纯化

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at –20 degree C. Stable for 1 year.

酶联免疫吸附测定, 免疫印迹

ELISA: 1:2000. Western Blot: 1:500

Immunogen: Clone (M121) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 397 of human FAK. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.

Buffer: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in signal transduction pathways important for cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr-397, which is a binding site for Src family kinases, PI3-Kinase, and PLC-gamma. The recruitment of Src family kinases results in the phosphorylation of tyrosine 407, 576, and 577 in the catalytic domain, and tyrosine 871 and 925 in the carboxy-terminal region of FAK. Thus, the phosphorylation of Tyr-397 is a critical step in the activation of FAK.

Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14:147. Parsons, J.T. et al. (2000) Oncogene 19:5606. Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14:1680. Schlaepfer, D.D. et al. (1994) Nature 372:786-791.

0.1毫升

380美元