semaphorin-3A; Semaphorin-3A; semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; OTTHUMP00000161379; OTTHUMP00000208884; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III
SEMA3A; SEMA3A; SemD; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243; SEMAD
The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera.
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Store at -20 degree C. Do not aliquot. Stable for 1 year.
ELISA: 1:2000. Western Blot: 1:1000
Immunogen: Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the C-terminal region of human Sema3A. The sequence used has two amino acid differences from rat and mouse Sema3A, and has low homology to other semaphorin family members.
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3Acontaining region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Kolodokin, A.L. et al. (1993) Cell 75:1389. Luo, Y. et al. (1993) Cell 75:217. Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
NCBI GI登录号 :
Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!CRMPs In Sema3A Signaling Pathway 119525!!SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway 119523!!Sema3A PAK Dependent Axon Repulsion Pathway 119524!!Semaphorin Interactions Pathway 119519
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq]
Function: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Subcellular location: Secreted. Domain: Strong binding to neuropilin is mediated by the carboxy third of the protein. Sequence similarities: Belongs to the semaphorin family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 PSI domain.Contains 1 Sema domain.
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