WASP / N-WASP; Neural Wiskott-Aldrich syndrome protein; WASL
neural Wiskott-Aldrich syndrome protein; Neural Wiskott-Aldrich syndrome protein; neural Wiskott-Aldrich syndrome protein; OTTHUMP00000211994; Wiskott-Aldrich syndrome gene-like; Wiskott-Aldrich syndrome-like
人类, 大鼠, 小鼠
This antibody detects a 63 kDa* protein corresponding to the molecular mass of WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, and SKN-SH cells, this antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP.
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Store at -20 degree C. Do not aliquot. Stable for 1 year.
ELISA: 1:2000. Western Blot: 1:1000
Immunogen: A synthetic peptide (coupled to KLH) corresponding to a region near the C-terminus of human WASP. This sequence is highly homologous with a similar region in human N-WASP.
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.
Cory, G.O. et al. (2003) Mol Cell. 11(5):1229-39. Higgs, H.N. & Pollard, T.D. (2001) Annu Rev Biochem 70:649-676.
NCBI GI登录号 :
Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); CDC42 Signaling Events Pathway (137994); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); DCC Mediated Attractive Signaling Pathway (161000); EGF Receptor (ErbB1) Signaling Pathway (137970)
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq]
Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Ref.11. Subunit structure: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11. By similarity. Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli EspF-like protein encoded on prophage U. Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF-like protein encoded on prophage U. Ref.4 Ref.5 Ref.6 Ref.11. Subcellular location: Cytoplasm cytoskeleton. By similarity. Nucleus. By similarity. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Sequence similarities: Contains 1 CRIB domain.Contains 1 WH1 domain.Contains 2 WH2 domains.
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San Diego, CA 92195-3308