产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗P16(小鼠和人类)小鼠单克隆抗体
目录 :
MBS475739
规格 :
0.1毫升
价格 :
335美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
[4G5-5A10-3G6]
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS475739 图像 1
Western blot analysis using P16 mouse mAb against truncated P16 recombinant protein.
图像 2 :
MyBioSource MBS475739 图像 2
Immunohistochemical analysis of paraffin-embedded rat liver tissue (A), human brain tumor (B), breast cancer (C), esophageal epithelium tissue (D), mouse brain tissue (E) and stomach tisue (F), showing nuclear localization using P16 mouse mAb with DAB sta
产品信息
目录号 :
MBS475739
产品类型 :
抗体
产品全称 :
抗P16(小鼠和人类)小鼠单克隆抗体
产品简称 :
[P16]
产品名称同义词 :
[P16]
其他名称 :
[p16, partial; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A; cyclin dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A]
产品基因名称 :
[P16]
产品基因名称同义词 :
[P16]
其他基因名称 :
[CDKN2A;CDKN2A;ARF;MLM;P14;P16;P19;CMM2;INK4;MTS1;TP16;CDK4I;CDKN2;INK4A;MTS-1;P14ARF;P19ARF;P16INK4;P16INK4A;P16-INK4A;CDK4I;MTS-1;p16-INK4;p16INK4A]
克隆性 :
单克隆
抗体亚型 :
小鼠IgG1
克隆 :
[4G5-5A10-3G6]
宿主 :
小鼠
反应物种 :
人类, 大鼠, 小鼠
纯度 :
腹水
形式 :
腹水含0.03% 叠氮化钠
储存稳定性 :
Store at +4°C short term. store at -20°c long term.Avoid freeze/thaw cycle
检测过的应用 :
免疫印迹(免疫印迹), 免疫组化(免疫组化)
应用笔记 :
WB:1/500-1/2000。 IHC:1/200-1/1000。 Elisa:1/10000
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
其它信息1 :
Antibody type: Primary antibody. Conjugation: Unconjugated. Modification: unmodified. Subcellular location: Mitochondrion,Nucleus
其他信息2 :
Immunogen: Purified recombinant fragment of P16 expressed in E Coli.
产品描述 :
The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.
NCBI GI登录号 :
89355819
NCBI登录号 :
ABD72255.1
NCBI信号通路 :
Apoptosis Pathway (198797); Apoptosis Modulation And Signaling Pathway (198822); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cellular Senescence Pathway (905991)
NCBI总结 :
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
UniProt数据库总结 :
p16-INK4A: a cell-cycle regulatory protein that interacts with CDK4 and CDK6, inhibiting their ability to interact with cyclins D. Inhibits the phosphorylation of the retinoblastoma protein by CDK4 or CDK6, and entry into the S phase of the cell cycle. The p16INK4A and p14ARF proteins are encoded by CDKN2A, a known tumour suppressor gene in multiple cancers. CDKN2A is inactivated in 72% of cases of lung squamous cell carcinoma: 21% by epigenetic silencing by methylation, 18% inactivating mutation, 4% by exon 1b skipping, and 29% by homozygous deletion. Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome, Li-Fraumeni syndrome, and the melanoma-astrocytoma syndrome. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma. Four alternatively spliced p16 isoforms have been reported. Two alternatively spliced isoforms of ARF have been reported. Protein type: Cell cycle regulation; Nucleolus; Tumor suppressor. Chromosomal Location of Human Ortholog: 9p21.3. Cellular Component: cytoplasm; cytosol; nucleus. Molecular Function: cyclin-dependent protein kinase inhibitor activity; NF-kappaB binding; protein binding; protein kinase binding. Biological Process: cell cycle arrest; G1/S transition of mitotic cell cycle; inhibition of NF-kappaB transcription factor; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cell-matrix adhesion; negative regulation of cyclin-dependent protein kinase activity; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; Ras protein signal transduction. Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 2; Melanoma-astrocytoma Syndrome; Melanoma-pancreatic Cancer Syndrome
尺寸1 :
0.1毫升
价格1 :
335美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。