产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Anti-Fibrillarin (Nop1p)
目录 :
MBS500003
规格 :
0.1毫升
价格 :
300美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
38F3
反应物种 :
酿酒酵母S288c, 人类, 大鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
产品信息
目录号 :
MBS500003
产品类型 :
抗体
产品全称 :
Anti-Fibrillarin (Nop1p)
产品简称 :
Fibrillarin
其他名称 :
Nop1p; rRNA 2'-O-methyltransferase fibrillarin; Nop1p; Histone-glutamine methyltransferase; U3 small nucleolar RNA-associated protein NOP1
产品基因名称 :
NOP1
其他基因名称 :
NOP1; NOP1; LOT3; LOT3; Nucleolar protein 1
UniProt数据库进入名 :
FBRL_YEAST
克隆性 :
单克隆
抗体亚型 :
IgG1
克隆 :
38F3
宿主 :
小鼠
反应物种 :
人类, 大鼠
序列长度 :
327
特异性 :
Specific for the ~34k Fibrillarin protein in Western Blots of human, rat, plant, Drosophila, C. elegans and S. pombe. It is expected that it will work on other mammal tissues.
纯度 :
总数IgG分馏成分
形式 :
液体。 含10 mM叠氮化钠
储存稳定性 :
Store at -20 degree C: stable for at least 1 year.
检测过的应用 :
免疫印迹(免疫印迹), 免疫荧光(IF)
应用笔记 :
Quality Control: Western blots performed on each lot. WB: 1:1,000. IF: 1:500
其它信息1 :
Antigen: Yeast nuclear preparations. Immunogen Information: Yeast nuclear preparations. Immunogen Species: Yeast. Packing: 100ul antibody per vial+10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.
其他信息2 :
Species Reactivity Note: The antibody has been directly tested for reactivity in human, rat, plant, Drosophila, C. elegans and S. pombe. It is expected that it will work on other mammal tissues. Biological Significance: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
产品描述 :
小鼠单克隆抗体
产品引用 :
Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123-133 (1985). Aris JP and Blobel G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107:17-31 (1988). Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 23:8519-8527 (2003). Tyagi S and Alsmadi O. Imaging native beta-actin mRNA in motile fibroblasts. Biophys J. 87:4153-62 (2004). Paeschke1 K, Simonsson T, Postberg J, Rhodes D, Lipps H-J. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo Nature Structural & Molecular Biology 12, 847-854 (2005).
NCBI GI登录号 :
6320190
NCBI登录号 :
NP_010270.1
NCBI基因登录号 :
NM_001180073.1
UniProt数据库登录号 :
P15646
NCBI信号通路 :
Ribosome Biogenesis In Eukaryotes Pathway (199271); Ribosome Biogenesis In Eukaryotes Pathway (199266)
UniProt数据库总结 :
Function: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (Ref.5). Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Involved in the biogenesis of the 18S rRNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (Ref.11). Catalytic activity: S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N(5)-methyl-L-glutamine-[histone]. Subunit structure: Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, NOP1, SIK1/NOP56 and NOP58, plus a guide RNA. Interacts with snoRNA U3. Interacts with MPP10, NOP58, SIK1 and SOF1. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.1 Ref.6 Ref.7 Ref.8. Subcellular location: Nucleus nucleolus. Note: Fibrillar region of the nucleolus. Ref.1 Ref.2 Ref.9. Post-translational modification: Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues. Ref.9. Sequence similarities: Belongs to the methyltransferase superfamily. Fibrillarin family.
尺寸1 :
0.1毫升
价格1 :
300美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。