酿酒酵母S288c, 人类, 大鼠
Nop1p; rRNA 2'-O-methyltransferase fibrillarin; Nop1p; Histone-glutamine methyltransferase; U3 small nucleolar RNA-associated protein NOP1
NOP1; NOP1; LOT3; LOT3; Nucleolar protein 1
Specific for the ~34k Fibrillarin protein in Western Blots of human, rat, plant, Drosophila, C. elegans and S. pombe. It is expected that it will work on other mammal tissues.
液体。 含10 mM叠氮化钠
Store at -20 degree C: stable for at least 1 year.
Quality Control: Western blots performed on each lot. WB: 1:1,000. IF: 1:500
Antigen: Yeast nuclear preparations. Immunogen Information: Yeast nuclear preparations. Immunogen Species: Yeast. Packing: 100ul antibody per vial+10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.
Species Reactivity Note: The antibody has been directly tested for reactivity in human, rat, plant, Drosophila, C. elegans and S. pombe. It is expected that it will work on other mammal tissues. Biological Significance: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123-133 (1985). Aris JP and Blobel G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107:17-31 (1988). Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 23:8519-8527 (2003). Tyagi S and Alsmadi O. Imaging native beta-actin mRNA in motile fibroblasts. Biophys J. 87:4153-62 (2004). Paeschke1 K, Simonsson T, Postberg J, Rhodes D, Lipps H-J. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo Nature Structural & Molecular Biology 12, 847-854 (2005).
NCBI GI登录号 :
Ribosome Biogenesis In Eukaryotes Pathway (199271); Ribosome Biogenesis In Eukaryotes Pathway (199266)
Function: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (Ref.5). Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Involved in the biogenesis of the 18S rRNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (Ref.11). Catalytic activity: S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N(5)-methyl-L-glutamine-[histone]. Subunit structure: Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, NOP1, SIK1/NOP56 and NOP58, plus a guide RNA. Interacts with snoRNA U3. Interacts with MPP10, NOP58, SIK1 and SOF1. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.1 Ref.6 Ref.7 Ref.8. Subcellular location: Nucleus nucleolus. Note: Fibrillar region of the nucleolus. Ref.1 Ref.2 Ref.9. Post-translational modification: Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues. Ref.9. Sequence similarities: Belongs to the methyltransferase superfamily. Fibrillarin family.
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