抗体

Anti-Neurofilament L (NF-L)

MBS500007

0.1毫升

300美元

单克隆

小鼠

未共轭

DA2

人类, 大鼠

免疫印迹, 免疫细胞化学

抗体

Anti-Neurofilament L (NF-L)

Neurofilament NF-L ms

Neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein

NEFL

NEFL;NEFL;NFL;NF-L;NF68;CMT1F;CMT2E;NF-L

NFL_PIG

单克隆

IgG1

DA2

小鼠

大鼠

549

Specific for the ~68k Neurofilament L protein.

Total IgG fraction (Unpurified, concentrated culture supernatant.)

液体含10mM叠氮化钠

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

免疫印迹(免疫印迹), 免疫荧光(IF)

Quality Control: Western blots performed on each lot. WB: 1:1,000. IF: 1:100

Antigen: Preparation of porcine intermediate filaments. Immunogen Information: Preparation of porcine NF-L. Immunogen Species: Pig

Reactivity Assumed Based on 100% Sequence Homology: Chicken, human, mouse. Species Reactivity Note: The antibody has been directly tested for reactivity in bovine, chicken, human, mouse and rat tissues. Biological Significance: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).

小鼠单克隆抗体

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.

143811426

P02547.3

P02547

68

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. Subunit structure: Interacts with ARHGEF28 . By similarity. Interacts with TRIM2 . By similarity. Domain: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. Post-translational modification: O-glycosylated . By similarity.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization . By similarity.Ubiquitinated in the presence of TRIM2 and UBE2D1 . By similarity. Miscellaneous: NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. Sequence similarities: Belongs to the intermediate filament family.

0.1毫升

300美元