glutamate receptor 1; Glutamate receptor 1; glutamate receptor 1; GluR1; gluR-1; gluR-K1; glutamate receptor A; glutamate receptor subunit GluR1; AMPA-selective glutamate receptor 1; glutamate receptor, ionotropic, AMPA1 (alpha 1); glutamate receptor, ionotropic, AMPA 1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic, AMPA 1
Gria1; Gria1; GluA1; gluR-A; Glur1; GluR-1; GluA1
Specific for the ~100k GluR1 protein in Western blots of rat brain extracts.
Protein G purified (Protein G purified culture supernatant)
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage at -80 degree C is recommended; Short term storage is -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for at least one year at -20 degree C.
Western Blot (WB). Immunohistochemistry (IHC) has not yet been determined.
Quality Control: Western blots performed on each lot. WB: 1:1000
Antigen: Peptide corresponding to amino acid residues from the N-terminal region of rat GluR1. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Immunogen Species: Rat
Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990;Hollmann and Heinemann, 1994).
Chung HJ, Steinberg JP, Huganir RL, Linden DJ (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755. Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108. Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560.
NCBI GI登录号 :
Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Circadian Entrainment Pathway (698787); Circadian Entrainment Pathway (699872); Dopaminergic Synapse Pathway (469193); Dopaminergic Synapse Pathway (469185); Glutamatergic Synapse Pathway (213815); Glutamatergic Synapse Pathway (213811)
mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning [RGD, Feb 2006]
Function: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Ref.12 Ref.17. Subunit structure: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with HIP1, RASGRF2, SYNDIG1 and LRFN1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain). Subcellular location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane postsynaptic density . By similarity. Cell projection dendrite . By similarity. Cell projection dendritic spine . By similarity. Note: Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Ref.11 Ref.12 Ref.17. Tissue specificity: Detected in cerebellum (at protein level). Ref.11. Domain: The M4 transmembrane segment mediates tetramerization and is required for cell surface expression . By similarity. Post-translational modification: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis . By similarity. Polymorphism: Both variants Ser-710 and Thr-710 are phosphorylated at this position. Miscellaneous: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate. Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. [View classification]
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