产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗磷酸化Tyr15 cdc2
目录 :
MBS502011
规格 :
0.1毫升
价格 :
345美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类, 大鼠
应用 :
免疫印迹
更多信息或购买 :
产品信息
目录号 :
MBS502011
产品类型 :
抗体
产品全称 :
抗磷酸化Tyr15 cdc2
产品简称 :
cdc2(Tyr15)
其他名称 :
cyclin-dependent kinase 1; Cyclin-dependent kinase 1; cyclin-dependent kinase 1; p34 protein kinase; cell division protein kinase 1; cell division cycle 2 homolog A; Cell division cycle control protein 2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
产品基因名称 :
CDK1
其他基因名称 :
Cdk1;Cdk1;Cdc2;Cdc2a;Cdc2;Cdc2a;Cdkn1;CDK1
UniProt数据库进入名 :
CDK1_RAT
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 蛙
序列长度 :
297
特异性 :
Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the lambda-phosphatase treatment.
纯度 :
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
形式 :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
储存稳定性 :
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
检测过的应用 :
免疫印迹(免疫印迹)
应用笔记 :
Quality Control: Western blots performed on each lot. WB: 1:1000
其它信息1 :
Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr15 of rat cdc2. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH. Immunogen Species: Rat
其他信息2 :
Reactivity Assumed Based on 100% Sequence Homology: Mouse, rat, zebra fish. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human and Xenopus tissue. It is anticipated that the antibody will also react with mouse, rat and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).
产品描述 :
亲和纯化兔多克隆抗体
产品引用 :
Dranovsky A, Vincent I, Gregori L, Schwarzman A, Colflesh D, Enghild J, Strittmatter W, Davies P, Goldgaber D (2001) cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer's disease pathology. Neurobiol Aging 22:517-528. Konishi Y, Bonni A (2003) The E2F-cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons. J Neurosci 23:1649-1658. Maller JL (1991) Mitotic control. Curr Opin Cell Biol 3:269-275. Nakamizo A, Inamura T, Inoha S, Amano T, Ochi H, Ikezaki K, Fukui M (2002) Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines. J Neurooncol 59:7-13. Okano HJ, Pfaff DW, Gibbs RB (1996) Expression of EGFR-, p75NGFR-, and PSTAIR (cdc2)-like immunoreactivity by proliferating cells in the adult rat hippocampal formation and forebrain. Dev Neurosci 18:199-209.
NCBI GI登录号 :
9506475
NCBI登录号 :
NP_062169.1
NCBI基因登录号 :
NM_019296.1
UniProt数据库登录号 :
P39951
NCBI分子量 :
34
NCBI信号通路 :
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (936554); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (936561); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (936560); ARMS-mediated Activation Pathway (936054); ATM Pathway (198524); Activated TLR4 Signalling Pathway (936709); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (936558); Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway (936539); Androgen Receptor Signaling Pathway (198529); Axon Guidance Pathway (936266)
NCBI总结 :
putative cell cycle control protein; homologous to S. pombe Cdc2 [RGD, Feb 2006]
UniProt数据库总结 :
Function: Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing . By similarity. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. Ref.4 Ref.5 Ref.6. Catalytic activity: ATP + a protein = ADP + a phosphoprotein.ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. Enzyme regulation: Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it . By similarity. Subunit structure: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cyclins-B (CCNB1, CCNB2 and CCNB3) to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Binds to RB1 and other transcription factors such as FOXO1 and RUNX2. Promotes G2-M transition when in complex with a cyclin-B. Interacts with DLGAP5. Binds to the CDK inhibitors CDKN1A/p21 and CDKN1B/p27. Isoform 2is unable to complex with cyclin-B1 and also fails to bind to CDKN1A/p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase . By similarity. Associates with cyclins-A and B1 during S-phase in regenerating hepatocytes. Interacts with FANCC . By similarity. Interacts with CEP63; this interaction recruits CDK1 to centrosomes . By similarity. Ref.4 Ref.6. Subcellular location: Nucleus. Cytoplasm . By similarity. Mitochondrion . By similarity. Cytoplasm cytoskeleton microtubule organizing center centrosome . By similarity. Note: Cytoplasmic during the interphase. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin B1. Accumulates in mitochondria in G2-arrested cells upon DNA- damage . By similarity. Ref.3 Ref.6. Induction: Follow a cyclic expression; during interphase, accumulates gradually following G1, S to reach a critical threshold at the end of G2, which promotes self-activation and triggers onset of mitosis. Induced transiently by TGFB1 at an early phase of TGFB1-mediated apoptosis. Expressed during S-phase in mitogen-stimulated hepatocytes. Ref.4 Ref.6. Post-translational modification: Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylation by PKMYT1 and WEE1 takes place during mitosis to keep CDK1-cyclin-B complexes inactive until the end of G2. By the end of G2, PKMYT1 and WEE1 are inactivated, but CDC25A and CDC25B are activated. Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. Phosphorylation by WEE2 is also required for metaphase II exit during egg activation to ensure exit from meiosis in oocytes and promote pronuclear formation. Phosphorylated at Tyr-4 by PKR/EIF2AK2 upon genotoxic stress. This phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint . By similarity.Polyubiquitinated upon genotoxic stress . By similarity. Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.
尺寸1 :
0.1毫升
价格1 :
345美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。