产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗磷酸化Ser845 GluR1
目录 :
MBS502063
规格 :
0.15毫升
价格 :
345美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
大鼠
更多信息或购买 :
产品信息
目录号 :
MBS502063
产品类型 :
抗体
产品全称 :
抗磷酸化Ser845 GluR1
产品简称 :
GluR1亚单位(Ser845)
其他名称 :
glutamate receptor 1; Glutamate receptor 1; glutamate receptor 1; GluR1; gluR-1; gluR-K1; glutamate receptor A; glutamate receptor subunit GluR1; AMPA-selective glutamate receptor 1; glutamate receptor, ionotropic, AMPA1 (alpha 1); glutamate receptor, ionotropic, AMPA 1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic, AMPA 1
产品基因名称 :
GRIA1
其他基因名称 :
Gria1; Gria1; GluA1; gluR-A; Glur1; GluR-1; GluA1
UniProt数据库进入名 :
GRIA1_RAT
克隆性 :
多克隆
宿主 :
反应物种 :
大鼠
序列长度 :
907
特异性 :
Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is s completely eliminated by treatment with lambda-Ptase.
纯度 :
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
形式 :
150 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
储存稳定性 :
For long term storage -80 degree C is recommended. Shorterm storage at -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 yr at -20 degree C.
检测过的应用 :
Western Blot (WB), Dot Blots (DB), Immunohistochemistry (IHC) has not yet been determined
应用笔记 :
WB: 1:1000. IHC: 1:1000 (frozen sections). Quality Control Test: Western Blot performed on each lot.
其它信息1 :
Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH. Immunogen Species: Rat
其他信息2 :
Reactivity Assumed Based on 100% Sequence Homology: Human, mouse, non-human primates. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as arning and memory (Lee at al., 2003; Esteban at al., 2003).
产品描述 :
亲和纯化兔多克隆抗体
产品引用 :
Banke TG, Bowie D, Lee HK, Huganir RL, Schousboe A, Traynelis SF (2000) Control of GluR1 AMPA receptor function by cAMP-dependent protein kinase. J Neurosci 20:89-102. Esteban JA, Shi SH, Wilson C, Nuriya M, Huganir RL, Malinow R (2003) PKA phosphorylation of AMPA receptor subunits controls synaptic trafficking underlying plasticity. Nature Neurosci 6:136-143. Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108. Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560. Lee HK, Takamiya K, Han JS, Man HY, Kim CH, Rumbaugh G, Yu S, Ding L, He C, Petralia RS, Wenthold RJ, Gallagher M, Huganir RL (2003) Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory. Cell 112(5):631-643.
NCBI GI登录号 :
29789269
NCBI登录号 :
NP_113796.1
NCBI基因登录号 :
NM_031608.1
UniProt数据库登录号 :
P19490
NCBI分子量 :
100
NCBI信号通路 :
Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Circadian Entrainment Pathway (698787); Circadian Entrainment Pathway (699872); Dopaminergic Synapse Pathway (469193); Dopaminergic Synapse Pathway (469185); Glutamatergic Synapse Pathway (213815); Glutamatergic Synapse Pathway (213811)
NCBI总结 :
mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning [RGD, Feb 2006]
UniProt数据库总结 :
Function: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Ref.12 Ref.17. Subunit structure: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with HIP1, RASGRF2, SYNDIG1 and LRFN1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain). Subcellular location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane postsynaptic density . By similarity. Cell projection dendrite . By similarity. Cell projection dendritic spine . By similarity. Note: Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Ref.11 Ref.12 Ref.17. Tissue specificity: Detected in cerebellum (at protein level). Ref.11. Domain: The M4 transmembrane segment mediates tetramerization and is required for cell surface expression . By similarity. Post-translational modification: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis . By similarity. Polymorphism: Both variants Ser-710 and Thr-710 are phosphorylated at this position. Miscellaneous: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate. Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. [View classification]
尺寸1 :
0.15毫升
价格1 :
345美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。