产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗NMDA受体, NR2B亚单位
目录 :
MBS502086
规格 :
0.01毫克
价格 :
345美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫沉淀
更多信息或购买 :
产品信息
目录号 :
MBS502086
产品类型 :
抗体
产品全称 :
抗NMDA受体, NR2B亚单位
产品简称 :
NMDA NR2B亚单位
其他名称 :
glutamate receptor ionotropic, NMDA 2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B
产品基因名称 :
GRIN2B
其他基因名称 :
Grin2b;Grin2b;GluN2B;GluN2B;NMDAR2B;NR2B
UniProt数据库进入名 :
NMDE2_RAT
克隆性 :
多克隆
宿主 :
反应物种 :
大鼠, 小鼠, 人类
序列长度 :
1482
特异性 :
Specific for the ~180k NR2B subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.
纯度 :
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
形式 :
10 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh
储存稳定性 :
Store at -20 degree C; stable for at least 1 year
检测过的应用 :
免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫沉淀(免疫沉淀)
应用笔记 :
Quality Control: Western blots performed on each lot. WB: 1:1000. IHC (frozen sections; unpublished observations): 1:1000 to 1:2000. IP: 3 ul per 200 ug lysate
其它信息1 :
Antigen: Fusion protein from the C-terminus of the NR2B subunit of rat NMDA receptor. Immunogen Information: Fusion protein from the C-terminal region of the NR2B subunit. Immunogen Species: Rat
其他信息2 :
Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue. Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).
产品描述 :
亲和纯化兔多克隆抗体
产品引用 :
Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025. Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. Clayton DA, Grosshans DR, Browning MD (2002a) Aging and surface expression of hippocampal NMDA receptors. J Biol Chem 277:14367-14369. Clayton DA, Mesches MH, Alvarez E, Bickford PC, Browning MD (2002b) A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat. J Neurosci 22:3628-3637. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
NCBI GI登录号 :
6980984
NCBI登录号 :
NP_036706.1
NCBI基因登录号 :
NM_012574.1
UniProt数据库登录号 :
Q00960
NCBI分子量 :
180
NCBI信号通路 :
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (936445); Alcoholism Pathway (585585); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (936452)
NCBI总结 :
subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]
UniProt数据库总结 :
Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death . By similarity. Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3. Interacts with HIP1 and Neto1 . By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with DAPK1 . By similarity. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Post-translational modification: Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity . By similarity. Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. [View classification]
尺寸1 :
0.01毫克
价格1 :
345美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。