抗体

抗突触I

MBS502117

0.01毫克

345美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠, 牛

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

抗体

抗突触I

突触I

突触1;突触1;突触1;突触I

SYN1

SYN1;SYN1

SYN1_BOVIN

多克隆

兔

人类, 小鼠, 大鼠

706

Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.)

10 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

免疫印迹(免疫印迹), 免疫荧光(IF), 免疫组化(免疫组化), 免疫沉淀(免疫沉淀)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: 1:1000. IF: 1:1000. IP: 1 ug per 200 ug lysate

Antigen: Native protein purified from bovine brain. Immunogen Information: Native protein purified from bovine brain. Immunogen Species: bovine

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat, mouse and human tissue. Biological Significance: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

亲和纯化兔多克隆抗体

Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380. Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953. Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437. Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162. Nayak AS, Moore CI, Browning MD (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456.

27806393

NP_776616.1

NM_174191.2

P17599

78

Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level . By similarity. Subunit structure: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ibinteracts with PRNP . By similarity. Subcellular location: Cell junction synapse. Golgi apparatus . By similarity. Domain: The A region binds phospholipids with a preference for negatively charged species . By similarity. Post-translational modification: Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles . By similarity. Sequence similarities: Belongs to the synapsin family.

0.01毫克

345美元