抗体

抗酪氨酸羟化酶

MBS502130

0.1毫升

345美元

多克隆

兔

未共轭

免疫印迹, 免疫组化, 免疫细胞化学

抗体

抗酪氨酸羟化酶

酪氨酸羟化酶

tyrosine 3-monooxygenase; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase

TH

Th; Th; The; TH

TY3H_RAT

多克隆

兔

All mammalian

498

Specific for the ~60k tyrosine hydroxylase protein.

Affinity Purified (Prepared from rabbit serum by affinity purification using a protein A column and using a column to which the immunogen was coupled.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

免疫印迹(免疫印迹), 免疫荧光(IF), 免疫组化(免疫组化)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC (frozen sections; Xu et al., 1998): 1:1000. IF (frozen sections; Xu et al., 1998): 1:1000

Antigen: SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma. Immunogen Information: SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma. Immunogen Species: Rat

Species Reactivity Note: The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF. Biological Significance: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).

亲和纯化兔多克隆抗体

Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem 91:1025-1043. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116. Xu ZQ, Lew JY, Harada K, Aman K, Goldstein M, Deutch A, Haycock JW, Hokfelt T (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci 82:727-738

6981652

NP_036872.1

NM_012740.3

P04177

60

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (935647); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198489); Catecholamine Biosynthesis Pathway (935648); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (434674); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Catecholamine Synthesis Pathway (198533)

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]

Function: Plays an important role in the physiology of adrenergic neurons. Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin. Cofactor: Fe2+ ion. Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity. Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. Subunit structure: Homotetramer. Post-translational modification: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

0.1毫升

345美元