抗体

抗磷酸化Ser40酪氨酸羟化酶

MBS502134

0.1毫升

345美元

多克隆

兔

未共轭

磷酸化

免疫印迹, 免疫组化, 免疫细胞化学

抗体

抗磷酸化Ser40酪氨酸羟化酶

酪氨酸羟化酶(Ser40)

tyrosine 3-monooxygenase; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase

TH

Th; Th; The; TH

TY3H_RAT

多克隆

兔

Many Mammalian/ Many Non-mammalian/

498

Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

免疫印迹(免疫印迹), 免疫荧光(IF), 免疫组化(免疫组化)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC (frozen sections; Witkovsky et al., 2000): 1:1000. IF (frozen sections; Witkovsky et al., 2000): 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH. Immunogen Species: Rat

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with many mammalian and non-mammalian species. Biological Significance: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

亲和纯化兔多克隆抗体

Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem 91:1025-1043. Haycock JW, Ahn NG, Cobb MH, Krebs EG (1992) ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci (USA) 89:2365- 2369. Haycock JW, Bennett WF, George RJ, Waymire JC (1982) Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem 257:13699-13703. Jedynak JP, Ali SF, Haycock JW, Hope BT (2002) Acute administration of cocaine regulates the phosphorylation of serine-19,-31 and-40 in tyrosine hydroxylase. J Neurochem 82:382-388. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.

6981652

NP_036872.1

NM_012740.3

P04177

60

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (935647); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198489); Catecholamine Biosynthesis Pathway (935648); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (434674); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Catecholamine Synthesis Pathway (198533)

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]

Function: Plays an important role in the physiology of adrenergic neurons. Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin. Cofactor: Fe2+ ion. Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity. Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. Subunit structure: Homotetramer. Post-translational modification: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

0.1毫升

345美元