抗体

抗微管相关蛋白2(微管相关蛋白2)

MBS502140

0.1毫升

300美元

多克隆

鸡

未共轭

免疫印迹, 免疫细胞化学

抗体

抗微管相关蛋白2(微管相关蛋白2)

微管相关蛋白2(微管相关蛋白2)

microtubule-associated protein 2 isoform 5; Microtubule-associated protein 2; microtubule-associated protein 2; MAP-2; microtubule-associated protein 2

微管相关蛋白2

微管相关蛋白2;微管相关蛋白2;MAP2A;MAP2B;MAP2C;地图-2

MTAP2_HUMAN

多克隆

鸡

Most Mammals

559

Specific for the ~ 280k MAP2 protein in Western Blots of rat brain homogenate.

Total IgY fraction

液体含10mM叠氮化钠

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

免疫印迹(免疫印迹), 免疫荧光(IF)

Quality Control Test:. Western blots performed on each lot. WB: 1:20,000. IF: 1:2,500

Antigen: Bovine MAP2 isolated from brain by the GTP microtubule cycling method. Immunogen Species: bovine. Packaging: 100ul antibody per vial +10 mM NaN3. Adequate amount of material to conduct 10-mini Wester Blots.

Species Reactivity Note: The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react with other mammalian tissues. Biological Significance: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).

鸡多克隆抗体

Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res Mol Brain Res. 26(1-2):218-224. Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6.

87578394

NP_001034627.1

NM_001039538.1

P11137

280

LKB1 Signaling Events Pathway (137957); MAPK Cascade Pathway (198834); SIDS Susceptibility Pathways (198901)

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Subcellular location: Cytoplasm cytoskeleton . Probable. Post-translational modification: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly . By similarity. MAP2A/c is phosphorylated. Isoform MAP2cis phosphorylated by FYN at Tyr-67. Ref.8. Sequence similarities: Contains 3 Tau/MAP repeats.

0.1毫升

300美元