产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Goat anti-human alpha2Macroglobulin (alpha2M), Whole IgG from antiserum
目录 :
MBS512010
规格 :
10毫克
价格 :
230美元
克隆性 :
多克隆
宿主 :
山羊
共轭标签 :
未共轭
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
MBS512010
产品类型 :
抗体
产品全称 :
Goat anti-human alpha2Macroglobulin (alpha2M), Whole IgG from antiserum
产品简称 :
阿尔法巨球蛋白
产品名称同义词 :
阿尔法巨球蛋白, 人类
其他名称 :
Alpha-2-macroglobulin; Alpha-2-macroglobulin; alpha-2-macroglobulin; alpha-2-M; OTTHUMP00000196829; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
其他基因名称 :
A2M;A2M;A2MD;CPAMD5;FWP007;S863-7;DKFZp779B086;CPAMD5
UniProt数据库进入名 :
A2 MG_HUMAN
克隆性 :
多克隆
宿主 :
山羊
特异性 :
This antibody is specific for alpha2macroglobulin as demonstrated by immunoelectrophoresis and ELISA.
纯度 :
Purified IgG. Vial containing ml of whole IgG, representing approximately 1 ml of antiserum. Total protein is 10 mg.
形式 :
Whole IgG, clear liquid.
浓度 :
IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4.
储存稳定性 :
Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.
应用笔记 :
Suitable for use as a source of antibodies to human alpha2M.
其它信息1 :
Immunogen: Human alpha2macroglobulin purified from plasma.
其他信息2 :
Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol. Neutralizing Activity: Not determined
产品描述 :
alpha2Macroglobulin (alpha2M) is a large proteinase inhibitor molecule of 718,000 daltons, consisting of 4 identical subunits of 185,000 each. Produced in hepatocytes and macrophages, plasma concentrations of alpha2M are typically 2 uM in adults, and as high as 6 uM in childhood. alpha2M has the ability to inhibit most enzymes from the serine, metallo, cysteine and aspartate subclasses. It is not a member of the SERPIN family of inhibitors but belongs to a class of proteins that include pregnancy zone protein (PZP) and the complement proteins C3, C4 and C5. These proteins contain regions of conserved sequence as well as one or more internal beta-cysteinyl-gamma- glutamyl thiolester bonds, which in the case of alpha2M are susceptible to cleavage by enzymes or by nucleophilic compounds such as methylamine or ammonium ions. Although the precise nature of the interactions is as yet unknown, it is generally thought that cleavage of a bait region within the alpha2M molecule by an enzyme leads to a conformational change, which then traps and/or covalently binds the enzyme1,2. The active site of the trapped enzyme is usually still intact and able to cleave small substrates, but is inaccessible to larger natural substrates. The conformational change induced also exposes receptor-binding regions within the molecule, which may be important in the clearance of alpha2M-enzyme complexes from the circulation. It is thought that the main role of alpha2M in vivo is that of a "backup" inhibitor and scavenger of proteinases in blood and in tissues3,4, but it has also been reported to participate in other physiological processes, including regulation of immune function1,2.
产品引用 :
1. Salvesen G, Pizzo SV; Proteinase Inhibitors: alpha-Macroglobulins, Serpins and Kunins; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 241-258, J.B. Lippincott Co., Philadelphia PA, USA, 1994. 2. Barrett J; alpha2Macroglobulin; Methods in Enzymology, 80, pp 737-754, 1981 Larsson LJ, Neuenschwander DE, Strickland DK; Reaction of Proteinases with alpha2Macroglobulin: Evidence for Alternate Reaction Pathways in the Inhibition of Trypsin; Biochemistry 28, pp 7636-7643, 1989. 3. Schmidt B, Mitchell L, Ofosu FA, Andrew M; alpha2Macroglobulin is an Important Progressive Inhibitor of Thrombin in Neonatal and Infant Plasma; Thromb Haemostas 62, pp 1074-1077, 1989. 4. Hoogendoorn H, Toh CH, Nesheim ME, Giles AR; alpha2Macroglobulin Binds and Inhibits Activated Protein C; Blood 78, pp2283-2290, 1991.
NCBI GI登录号 :
112911
NCBI登录号 :
P01023.1
NCBI基因登录号 :
P01023
UniProt数据库登录号 :
P01023
NCBI分子量 :
163,291 Da
NCBI信号通路 :
Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Formation Of Platelet Plug Pathway (106029); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Intrinsic Pathway (106059); Lipid Digestion, Mobilization, And Transport Pathway (106111)
NCBI总结 :
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq]
UniProt数据库总结 :
Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Subunit structure: Homotetramer; disulfide-linked. Ref.8 Ref.10. Subcellular location: Secreted Ref.8. Tissue specificity: Secreted in plasma. Ref.8. Developmental stage: Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]. Sequence caution: The sequence AAT02228.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAD92851.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
尺寸1 :
10毫克
价格1 :
230美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。