蛋白

STK9重组蛋白

MBS515242

0.02毫克

270美元

重组蛋白

STK9重组蛋白

[STK9]

[CDKL5;EIEE2;ISSX]

[Homo sapiens cyclin-dependent kinase-like 5 (CDKL5), transcript variant I, mRNA; Cyclin-dependent kinase-like 5; cyclin-dependent kinase-like 5; serine/threonine kinase 9; serine/threonine-protein kinase 9; cyclin dependent kinase 5 transcript; cyclin-dependent kinase-like 5; Serine/threonine-protein kinase 9]

[STK9]

[CDKL5;EIEE2;ISSX]

[CDKL5;CDKL5;ISSX;STK9;EIEE2;STK9]

CDKL5_HUMAN

Sf9昆虫细胞

[1-498]

3434

>85%

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

0.1微克/微升

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Ships with Dry Ice.

免疫印迹(免疫印迹)

十二烷基磺酸钠-PAGE

Source Note: Recombinant human STK9 (CDKL5) (1-498) was expressed by baculovirus in Sf9 insect cells

Cellular Proteins; Signaling Proteins - Cellular Proteins

Recombinant human STK9 (CDKL5) (1-498) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics (1). The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder (2).

1. Ricciardi, S. et.al: CDKL5 influences RNA splicing activity by its association to the nuclear speckle molecular machinery. Hum. Molec. Genet. 18: 4590-4602, 2009. 2. Archer, H. L. et.al: CDKL5 mutations cause infantile spasms, early onset seizures, and severe mental retardation in female patients. J. Med. Genet. 43: 729-734, 2006.

83367066

NP_003150.1

NM_003159

O76039

~84 kDa

BDNF Signaling Pathway (712093)

This gene is a member of Ser/Thr protein kinase family and encodes a phosphorylated protein with protein kinase activity. Mutations in this gene have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Function: Mediates phosphorylation of MECP2. Ref.7 Ref.8. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Subunit structure: Interacts with MECP2. Ref.7. Subcellular location: Nucleus Ref.8. Tissue specificity: Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis. Post-translational modification: Autophosphorylated. Ref.7 Ref.8. Involvement in disease: Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).Epileptic encephalopathy, early infantile, 2 (EIEE2) [MIM:300672]: A severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients with epileptic encephalopathy early infantile type 2 manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements. However, EIEE2 and Rett syndrome are considered two distinct entities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.6 Ref.7 Ref.8 Ref.13 Ref.14 Ref.15 Ref.16 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23. Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain. Caution: It is uncertain whether Met-1 or Met-10 is the initiator. Sequence caution: The sequence CAA61445.1 differs from that shown. Reason: Frameshift at position 415.

0.02毫克

270美元

0.05毫克

450