抗体

抗大鼠CD8a PE-Cy5(克隆OX-8)(小鼠IgG1)

MBS520546

0.1毫克

680美元

单克隆

小鼠

PE-Cy5

OX-8

人类, 大鼠

抗体

抗大鼠CD8a PE-Cy5(克隆OX-8)(小鼠IgG1)

CD8a PE-Cy5

CD8a PE-Cy5(克隆OX-8)(小鼠IgG1);PE-Cy5抗大鼠CD8单克隆抗体

CD8a molecule; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2

CD8a PE-Cy5

CD8A;CD8A;CD8;MAL;p32;Leu2;MAL

CD8A_HUMAN

单克隆

小鼠IgG1

OX-8

小鼠

大鼠

235

CD8a(大鼠CD8a)

PE-Cy5

Antibody Concentration: 0.2 mg/ml

Store at 4 degree C. DO NOT FREEZE. Avoid prolonged exposure to light. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Check label for expiry date.

免疫组化(免疫组化)

Presentation: 100 ug PE-Cy5 conjugated Ig buffered in PBS, 0.09% sodium azide (NaN3) and 0.5% BSA as as stabilizer.

anti-rat CD8a monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine. (1,2,3,4.) The antigen recognized is a complex of surface glycoproteins of Mr 34,000, 39,000 and 76,000 Da and is the rat homologue of the human CD8 and the mouse Ly-2,3 antigen. (5,8) MBS520546 labels all peripheral T cells that are unlabelled by the (W3/25) monoclonal antibody. It labels a T-cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor. (2) MBS520546 and can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft vs. host disease. (7) This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being (W3/13) which labels all T cells, as well as (W3/25) and MBS520546(MRC 0X-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. Applications Include: Flow cytometry

19344022

AAH25715.1

P01732

25,729 Da

Adaptive Immune System Pathway 366160!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Downstream Signaling In Naive CD8+ T Cells Pathway 138018!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!IL12-mediated Signaling Events Pathway 137922!!Immune System Pathway 106386

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not . By similarity. Involvement in disease: Familial CD8 deficiency (CD8 deficiency) [MIM:608957]: Novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

0.1毫克

680美元