抗体

兔抗人类aFGF(成纤维细胞生长因子-1)多克隆

MBS551008

0.1毫克

280美元

多克隆

兔

未共轭

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

兔抗人类aFGF(成纤维细胞生长因子-1)多克隆

aFGF(成纤维细胞生长因子-1)

Rabbit anti Human aFGF (FGF1) Polyclonal purified; afgf; fibrobalst growth factor; polyclonal; pab; FGF1; FGF-acidic; fgf acidic

fibroblast growth factor 1 isoform 1; Fibroblast growth factor 1; Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1

aFGF

成纤维细胞生长因子-1;FGFA;FGF-1;aFGF;ECGF;HBGF-1

FGF1_HUMAN

多克隆

IgG

兔

人类

155

Serum IgG fraction was purified by Protein A affinity chromatography.

0.2 um filtered solution in PBS with 5% trehalose.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA)

ELISA: Use at a concentration range of 0.5-1 ug/ml. The detection limit for FGF-acidic is approximately 0.15 ng/well. Western blot: A suitable range of concentrations of this antibody for WB detection is 0.125-0.5 ug/ml.

Source Note: Polyclonal aFGF antibody was produced from sera of rabbits immunized with recombinant human fibroblast growth factor acidic.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers.

多克隆

aFGF and bFGF display 55% homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.

4503697

NP_000791.1

NM_000800.4

P05230

17,460 Da

Downstream Signaling Of Activated FGFR Pathway 160957!!FGF Signaling Pathway 137989!!FGFR Ligand Binding And Activation Pathway 106344!!FGFR1 Ligand Binding And Activation Pathway 106345!!FGFR1b Ligand Binding And Activation Pathway 106346!!FGFR1c Ligand Binding And Activation Pathway 106347!!FGFR2 Ligand Binding And Activation Pathway 106349!!FGFR2b Ligand Binding And Activation Pathway 106350!!FGFR2c Ligand Binding And Activation Pathway 106351!!FGFR3 Ligand Binding And Activation Pathway 106352

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Ref.20 Ref.23 Ref.40. Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu2+-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1 . By similarity. Interacts with S100A13. Interacts with LRRC59. Interacts with CSNKA, CSNKB and FIBP. While binding with LRRC59, CSNKA and FIBP seem mutually exclusive, CSNKB and FIBP may cooperatively interact with FGF1. Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.40. Subcellular location: Secreted. Cytoplasm. Cytoplasm cell cortex. Cytoplasm cytosol. Nucleus. Note: Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu2+ ions and S100A13. Secreted in a complex with SYT1 . By similarity. Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. Ref.21 Ref.22 Ref.24 Ref.25 Ref.28. Tissue specificity: Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. Ref.7 Ref.22. Post-translational modification: In the nucleus, phosphorylated by PKC/PRKCD. Ref.28. Sequence similarities: Belongs to the heparin-binding growth factors family.

0.1毫克

280美元