抗体

兔抗GM-CSF多克隆

MBS551019

0.1毫克

280美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

兔抗GM-CSF多克隆

GM-CSF

Rabbit anti GM-CSF Polyclonal purified; GM-CSF; gmcsf; csf2; Granulocyte Macrophage-Colony Stimulating Factor; polyclonal; pab

granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; CSF; Molgramostin; Sargramostim

GM-CSF

CSF2;GMCSF;GM-CSF;CSF

CSF2_HUMAN

多克隆

IgG

兔

小鼠, 大鼠

144

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

免疫印迹(免疫印迹), 免疫组化(免疫组化), 酶联免疫吸附测定(EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.

Source Note: Polyclonal GM-CSF antibody was produced from sera of rabbits immunized with synthetic protein containing GM-CSF antigenic determinants.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

多克隆

GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non- glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T- cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM- CSF.

GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions . J. Exp. Med., Sep 2009; 206: 2141 - 2149. the transcription factors stat5a/b regulate GM-CSF -mediated granulopoiesis . Blood, Sep 2009; 10.1182/blood-2009-04-216390. GM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace) . Blood, Sep 2009; 10.1182/blood-2009-04-215020. biologic activity of irradiated, autologous, GM-CSF -secreting leukemia cell vaccines early after allogeneic stem cell transplantation . PNAS, Sep 2009; 106: 15825 - 15830. stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor ( GM-CSF ) . Hum. Reprod., Sep 2009; 10.1093/humrep/dep307.

27437030

NP_000749.2

NM_000758.3

P04141

16,295 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Fc Epsilon RI Signaling Pathway (83082); Fc Epsilon RI Signaling Pathway (493)

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity. Biological Process: negative regulation of cytolysis; epithelial fluid transport; macrophage activation; embryonic placenta development; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication

0.1毫克

280美元

0.2毫克

385