抗体

兔抗P53多克隆

MBS551070

0.1毫克

280美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

兔抗P53多克隆

P53

兔抗P53多克隆纯化;P53;多克隆

cellular tumor antigen p53 isoform a; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

P53

TP53;P53

P53_HUMAN

多克隆

IgG

兔

人类, 小鼠, 大鼠, 兔

393

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.

The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C. Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. Shelf Life: >12 months

免疫印迹(免疫印迹), 免疫组化(免疫组化), 酶联免疫吸附测定(EIA)

ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of PEFF Human endometrial cancer tissue expressing P53.

Source Note: Polyclonal P53 antibody was produced from sera of rabbits immunized with synthetic protein containing P53 antigenic determinants.

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

多克隆

akt activation emulates chk1 inhibition and BCL2 overexpression and abrogates g2 cell cycle checkpoint by inhibiting brca1 foci . J. Biol. Chem., May 2010; 10.1074/jbc.M110.104372. ifn restores breast cancer sensitivity to fulvestrant by regulating stat1, ifn regulatory factor 1, nf-b, BCL2 family members, and signaling to caspase-dependent apoptosis . Mol. Cancer Ther., May 2010; 9: 1274 - 1285. analysis of pro-apoptotic BCL2 family member initiators bim, bid, and bbc3 in retinal ganglion cell death . Invest. Ophthalmol. Vis. Sci., Apr 2010; 51: 2118. BCL2 and human papilloma virus 16 as predictors of outcome following concurrent chemoradiation for advanced oropharyngeal cancer . Clin. Cancer Res., Apr 2010; 16: 2138 - 2146. BCL2 is not required for the development and maintenance of leukaemia stem cells in mice . Carcinogenesis, Mar 2010; 10.1093/carcin/bgq062.

120407068

NP_000537.3

NM_000546.5

P04637

43,653 Da

Activation Of BH3-only Proteins Pathway (105658); Activation Of NOXA And Translocation To Mitochondria Pathway (105660); Activation Of PUMA And Translocation To Mitochondria Pathway (105661); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198806); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648)

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]

p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. Protein type: DNA-binding; Motility/polarity/chemotaxis; Tumor suppressor; Nuclear receptor co-regulator; Transcription factor; Activator. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: PML body; transcription factor TFIID complex; protein complex; nuclear matrix; mitochondrion; endoplasmic reticulum; replication fork; cytosol; nucleoplasm; nuclear body; mitochondrial matrix; nuclear chromatin; cytoplasm; nucleolus; chromatin; nucleus. Molecular Function: identical protein binding; protease binding; protein phosphatase 2A binding; zinc ion binding; p53 binding; protein N-terminus binding; receptor tyrosine kinase binding; transcription factor binding; protein phosphatase binding; protein kinase binding; histone acetyltransferase binding; protein binding; histone deacetylase regulator activity; copper ion binding; enzyme binding; DNA binding; protein heterodimerization activity; chaperone binding; ubiquitin protein ligase binding; damaged DNA binding; chromatin binding; transcription factor activity; ATP binding. Biological Process: viral reproduction; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; gastrulation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to antibiotic; regulation of apoptosis; cellular response to glucose starvation; protein localization; negative regulation of neuroblast proliferation; base-excision repair; transforming growth factor beta receptor signaling pathway; cerebellum development; protein complex assembly; cell cycle arrest; ER overload response; response to X-ray; somitogenesis; release of cytochrome c from mitochondria; chromatin assembly; cell aging; circadian behavior; rRNA transcription; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of fibroblast proliferation; negative regulation of DNA replication; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; negative regulation of transcription, DNA-dependent; regulation of tissue remodeling; negative regulation of apoptosis; transcription from RNA polymerase II promoter; G1 DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator; apoptosis; negative regulation of transcription from RNA polymerase II promoter; response to salt stress; entrainment of circadian clock by photoperiod; positive regulation of protein oligomerization; negative regulation of cell proliferation; positive regulation of histone deacetylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; T cell proliferation during immune response; positive regulation of neuron apoptosis; double-strand break repair; response to gamma radiation; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; mitochondrial DNA repair; Notch signaling pathway; in utero embryonic development; B cell lineage commitment; multicellular organism growth; cell proliferation; neuron apoptosis; T cell lineage commitment; negative regulation of helicase activity; nucleotide-excision repair; protein import into nucleus, translocation; DNA strand renaturation; Ras protein signal transduction; negative regulation of cell growth; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus. Disease: Papilloma Of Choroid Plexus; Pancreatic Cancer; Nasopharyngeal Carcinoma; Li-fraumeni Syndrome 1; Breast Cancer; Osteogenic Sarcoma; Colorectal Cancer; Adrenocortical Carcinoma, Hereditary; Glioma Susceptibility 1; Hepatocellular Carcinoma; Basal Cell Carcinoma, Susceptibility To, 7

0.1毫克

280美元

0.2毫克

385