产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
Human basic Fibroblast Growth Factor Recombinant
目录 :
MBS553055
规格 :
0.01毫克
价格 :
145美元
更多信息或购买 :
产品信息
目录号 :
MBS553055
产品类型 :
重组蛋白
产品全称 :
Human basic Fibroblast Growth Factor Recombinant
产品简称 :
basic Fibroblast Growth Factor
产品名称同义词 :
fgf-2; fibrobalst growth factor; fgf2; bfgf
其他名称 :
fibroblast growth factor 2; Fibroblast growth factor 2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2
产品基因名称 :
bFGF
其他基因名称 :
纤维生长因子2;FGFB;FGF-2;bFGF;HBGF-2
UniProt数据库进入名 :
FGF2_HUMAN
宿主 :
大肠杆菌
序列长度 :
288
序列 :
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
纯度 :
>96%, as determined by SDS-PAGE and HPLC
形式 :
Human Basic FGF was lyophilized from a 0.2 um filtered solution in 1M NaCl,20mM PB pH 7.0.
储存稳定性 :
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
其它信息1 :
Domain: HBGF/IL1. Host Note: Optimized DNA sequence encoding Human basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
其他信息2 :
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Native human FGF basic is generated by the proteolytic removal of the signal peptide. and propeptide,the molecule has a calulated mass of 17 kDa. Recombinant bFGF is a disulfide-linked homodimeric protein consisting of 155 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
产品种类 :
细胞因子
产品描述 :
basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.
产品引用 :
expression of bFGF and il-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 545. p53 is necessary for bFGF -induced lens differentiation . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4369. regulation of basic fibroblast growing factor ( bFGF ) by retinal glial (m ller) cells under severe retinal ischemia . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2926. natural history of progressive experimental cnv in the rabbit after sustained release of both vegf and bFGF within the supra-choroidal space . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2970. keratocyte-associated mt1-mmp modulates bFGF -induced vegf expression . Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4967.
NCBI GI登录号 :
153285461
NCBI登录号 :
NP_001997.5
NCBI基因登录号 :
NM_002006.4
UniProt数据库登录号 :
P09038
NCBI分子量 :
30,770 Da
NCBI信号通路 :
Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Downstream Signaling Of Activated FGFR Pathway 160957!!Endochondral Ossification Pathway 198812!!FGFR Ligand Binding And Activation Pathway 106344!!FGFR1 Ligand Binding And Activation Pathway 106345!!FGFR1b Ligand Binding And Activation Pathway 106346!!FGFR1c Ligand Binding And Activation Pathway 106347!!FGFR2 Ligand Binding And Activation Pathway 106349!!FGFR2c Ligand Binding And Activation Pathway 106351!!FGFR3 Ligand Binding And Activation Pathway 106352
UniProt数据库总结 :
Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Ref.11 Ref.20. Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25. Subcellular location: Secreted. Nucleus. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. Ref.25 Ref.28. Tissue specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. Ref.11 Ref.18. Post-translational modification: Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. Miscellaneous: This protein binds heparin more strongly than does aFGF. Sequence similarities: Belongs to the heparin-binding growth factors family. Biophysicochemical propertiespH dependence:Retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees Celsius. Ref.11Temperature dependence:Inactivated after 3 minutes at 60 degrees Celsius or 1 minute at 80 degrees Celsius. Sequence caution: The sequence AAA52448.1 differs from that shown. Reason: Frameshift at positions 25, 82, 98 and 133. The sequence AAB21432.2 differs from that shown. Reason: Frameshift at position 25. The sequence AAB21432.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence ABO43041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABO43041.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA28027.1 differs from that shown. Reason: Frameshift at positions 25 and 102. The sequence CAA28027.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA73868.1 differs from that shown. Reason: Frameshift at position 25. The sequence CAA73868.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence EAX05222.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence EAX05222.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.
规格 :
0.01毫克
价格 :
145美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。