蛋白

人类白细胞介素1β重组

MBS553058

0.01毫克

220美元

重组蛋白

人类白细胞介素1β重组

白细胞介素1β

interleukin-1; hbgf; heparin binding; il1b; il-1 beta; il1-beta; il1-b; il-1b

interleukin-1 beta proprotein; Interleukin-1 beta; Catabolin

IL-1

IL1B;IL1F2;IL-1乙

IL1B_HUMAN

大肠杆菌

269

MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL RISDHHYSKG FRQAASVVVA MDKLRKMLVP CPQTFQENDL STFFPFIFEE EPIFFDTWDN EAYVHDAPVR SLNCTLRDSQ QKSLVMSGPY ELKALHLQGQ DMEQQVVFSM SFVQGEESND KIPVALGLKE KNLYLSCVLK DDKPTLQLES VDPKNYPKKK MEKRFVFNKI EINNKLEFES AQFPNWYIST SQAENMPVFL GGTKGGQDIT DFTMQFVSS

>97%, as determined by SDS-PAGE and HPLC

IL-1 beta was lyophilized from a 0.2 um filtered PBS pH 7.0.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: heparin binding growth factor. Host Note: Optimized DNA sequence encoding Human Interleukin-1 beta mature chain was expressed in Escherichia Coli.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Molecular Weight Note: Mature Human IL-1 beta, is generated by the proteolytic removal of the signal peptide. and propeptide.The molecule has a calculated molecular mass of approximately 17 kDa. Recombinant IL-1 beta is a disulfide-linked monomer protein consisting of two 154 amino acid residue subunits. Recombinant IL-1 beta migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

细胞因子

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

azithromycin and clarithromycin inhibit lipopolysaccharide-induced murine pulmonary neutrophilia mainly through effects on macrophage-derived granulocyte-macrophage colony-stimulating factor and interleukin-1 . J. Pharmacol. Exp. Ther., Oct 2009; 331: 104 - 113. high sensitivity of -cell replication to the inhibitory effects of interleukin-1 : modulation by adenoviral overexpression of igf2 in rat islets . J. Endocrinol., Oct 2009; 203: 55 - 63. bacillus anthracis capsule activates caspase-1/ice and induces interleukin-1 release from differentiated thp-1 and human monocyte-derived dendritic cells. . Infect. Immun., Sep 2009; 10.1128/IAI.00956-09. Interleukin-1 beta regulates proximal tubular cell transforming growth factor beta-1 signalling . Nephrol. Dial. Transplant., Sep 2009; 24: 2655 - 2665. ccaat enhancer binding protein- regulates matrix metalloproteinase-1 expression in interleukin-1 stimulated a549 lung carcinoma cells . Mol. Cancer Res., Sep 2009; 7: 1517 - 1524.

10835145

NP_000567.1

NM_000576.2

P01584

30,748 Da

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Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Ref.16. Subunit structure: Monomer. Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. Sequence similarities: Belongs to the IL-1 family.

0.01毫克

220美元