蛋白

大鼠PDGFR乙重组

MBS553313

0.05毫克

220美元

重组蛋白

大鼠PDGFR乙重组

[PDGFR乙]

[Rat CD140b Recombinant (PDGF Receptor beta) ; cd-140 PDGFRB; CD140B; MGC74795; PDGFR2; Rhe-PDGFRB PDGF Receptor alpha; pdgf-r]

[platelet-derived growth factor receptor beta; Platelet-derived growth factor receptor beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1]

[CD140b]

[PDGF-R]

[Pdgfrb; Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1]

PGFRB_RAT

1097

>95%, as determined by SDS-PAGE and HPLC

Recombinant PDGFR beta is supplied as a 0.2 um filtered PBS solution, pH7.2.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree to 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Domain: Ig-like C2. Source: HEk293 Cells. Source Note: Optimized DNA sequence encoding extracellular domain of rat PDGFR beta ncluding a C-terminal 6His tag was expressed in HEK293 cells.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Molecular Weight Note: Recombinant rat PDGF-R beta is a monomer protein consisting of 510 amino acid residue subunits, due to glycosylation migrates as an approximately 100 kDa protein on SDS-PAGE.

受体Coding sequence蛋白质编码序列

The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (PDGFA, PDGFB, PDGFC, and PDGFD. Ligand binding induces receptor dimerization, enabling autophosphorylation of specific tyrosine residues and subsequent recruitment of a variety of signal transduction molecules. PDGFR regulates normal cellular growth and differentiation, and expression of activated PDGFR promotes oncogenic transformation, suggesting that activating mutations or gene rearrangements could play a role in human tumorigenesis. Numerous in vitro and in vivo studies showed that inhibition of PDGFRA signaling disrupts cancer cell survival in the subset of GISTs with activating PDGFRA mutations.

hydrophobic matching controls the tilt and stability of the dimeric platelet-derived growth factor receptor (pdgfr) transmembrane segment . J. Biol. Chem., Jul 2012; 287: 26178 - 26186. pdgfr expression and function in fibroblasts derived from pluripotent cells is linked to dna demethylation . J. Cell Sci., May 2012; 125: 2276 - 2287. pdgf-induced migration of vascular smooth muscle cells is inhibited by heme oxygenase-1 via vegfr2 upregulation and subsequent assembly of inactive vegfr2/pdgfr heterodimers . Arterioscler Thromb Vasc Biol, May 2012; 32: 1289 - 1298. a viable mouse pdgfr-beta mutant exhibits cns-restricted pericyte deficiency and blood brain barrier defects . Invest. Ophthalmol. Vis. Sci., Mar 2012; 53: 2558. the leukemia-associated fusion protein tel-platelet-derived growth factor receptor (tel-pdgfr ) inhibits transcriptional repression of ptpn13 gene by interferon consensus sequence binding protein (icsbp) . J. Biol. Chem., Mar 2012; 287: 8110 - 8125.

29789275

NP_113713.1

NM_031525.1

Q05030

122,828 Da

binds platelet derived growth factor BB homodimer [RGD, Feb 2006]

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. Protein type: Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Kinase, protein; TK group; PDGFR family

0.05毫克

220美元

0.2毫克

595

1毫克

2220