人类, 小鼠, 大鼠
免疫印迹, 免疫组化, 免疫细胞化学
图像 1 :
Immunofluorescent analysis of rat brain section stained with chicken antibody to tyrosine hydroxylase, MBS555890, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. MBS555890 stains the dopaminergic TH-positive neurons and their processes of substantia nigra
[tyrosine 3-monooxygenase isoform b; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; tyrosine hydroxylase; Tyrosine 3-hydroxylase]
人类, 小鼠, 大鼠
Purified antibody at 1 mg/ml in 50% glycerol plus 5mM of Sodium Azide
Antibody can also be aliquotted and stored frozen at -20°C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8°C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
免疫细胞化学(免疫细胞化学), 免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF)
Immunofluorescence: 1:5,000. Immunohistochemistry: 1:5,000. Immunocytochemistry: 1:5,000. Western Blot: 1:50,000. Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Immunogen Sequence: Full length human tyrosine hydroxylase as expressed in and purified E. coli
Tyrosine Hydroxylase (TH) is a vital enzyme in the generation of L-DOPA from the amino acid tyrosine. TH expression is restricted in the brain, but expressed highly in locations where it is found. TH antibody are useful in identifying chatecholaminergic neurons. This antibody was made against full length human recombinant human tyrosine hydroxylase.
NCBI GI登录号 :
ATF-2 Transcription Factor Network Pathway (138006); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Alpha-synuclein Signaling Pathway (137913); Amine-derived Hormones Pathway (160983); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357)
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described. Protein type: Oxidoreductase; Endoplasmic reticulum; Mitochondrial; Vesicle; Amino Acid Metabolism - tyrosine; EC 126.96.36.199. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus. Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity. Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; eye photoreceptor cell development; organ morphogenesis; response to ethanol; circadian sleep/wake cycle; response to zinc ion; cerebral cortex development; response to activity; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to salt stress; response to estradiol stimulus; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; learning; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis. Disease: Segawa Syndrome, Autosomal Recessive
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San Diego, CA 92195-3308