产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Mouse anti actin alpha-skeletal
目录 :
MBS570258
规格 :
0.1毫克
价格 :
415美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
[3B3]
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS570258 图像 1
Paraffin sections of human striated skeletal muscle (above) and striated muscle (below) from human tongue immunostained with 3B3 (1:250)
图像 2 :
MyBioSource MBS570258 图像 2
产品信息
目录号 :
MBS570258
产品类型 :
抗体
产品全称 :
Mouse anti actin alpha-skeletal
产品简称 :
[actin alpha-skeletal]
其他名称 :
[肌动蛋白, 甲骨骼肌;肌动蛋白, 甲骨骼肌;肌动蛋白, 甲骨骼肌;杆状体肌病类型3;肌动蛋白, 甲1, 骨骼肌;阿尔法肌动蛋白]
其他基因名称 :
[ACTA1;ACTA1;ACTA;ASMA;CFTD;MPFD;NEM1;NEM2;NEM3;CFTD1;CFTDM;ACTA]
UniProt数据库进入名 :
ACTS_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG1
克隆 :
[3B3]
宿主 :
小鼠
反应物种 :
Caprine, Goat, Human, Rabbit, Rat, Swine.
特异性 :
Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.
形式 :
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
储存稳定性 :
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
检测过的应用 :
酶联免疫吸附测定, 免疫印迹 , 免疫组化(冷冻), 免疫组化(石蜡), 西方墨点法
应用笔记 :
3B3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titRation; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations.
image1头 :
试验数据
image2头 :
试验数据
其它信息1 :
Source Note: 3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
其他信息2 :
Reactivity Note: The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
产品种类 :
Cytoskeletal, Cardiovascular, Cell differentiation, Cytoskeleton, Stem cell research
产品描述 :
Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal alpha-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development alpha-cardiac actin becomes the main isoform in the myocardium, alpha-skeletal actin might still make up for almost half of its total striated muscle actin content.
产品引用 :
Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.
NCBI GI登录号 :
4501881
NCBI登录号 :
NP_001091.1
NCBI基因登录号 :
NM_001100.3
UniProt数据库登录号 :
P68133
NCBI分子量 :
42,051 Da
NCBI信号通路 :
Caspase Cascade In Apoptosis Pathway (137974); Hypothetical Network For Drug Addiction Pathway (198776); Myometrial Relaxation And Contraction Pathways (198759); RhoA Signaling Pathway (169356); Signaling Events Mediated By Focal Adhesion Kinase Pathway (169348); Striated Muscle Contraction Pathway (198903)
NCBI总结 :
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family. Protein type: Cell development/differentiation; Contractile; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q42.13. Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton. Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding. Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to steroid hormone stimulus; response to mechanical stimulus; muscle thin filament assembly; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding. Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion
尺寸1 :
0.1毫克
价格1 :
415美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。