产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
Basic Fibroblast Growth Factor (b-FGF)
目录 :
MBS590001
规格 :
96 Wells
价格 :
580美元
更多信息或购买 :
产品信息
目录号 :
MBS590001
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
Basic Fibroblast Growth Factor (b-FGF)
产品简称 :
Basic Fibroblast Growth Factor
其他名称 :
basic fibroblast growth factor, partial; Fibroblast growth factor 2; fibroblast growth factor 2; basic fibroblast growth factor bFGF; heparin-binding growth factor 2; prostatropin; fibroblast growth factor 2 (basic); Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
产品基因名称 :
b-FGF
其他基因名称 :
纤维生长因子2;纤维生长因子2;BFGF;FGFB;FGF-2;HBGF-2
UniProt数据库进入名 :
FGF2_HUMAN
序列长度 :
114
特异性 :
This sandwich ELISA recognises both natural and recombinant human bFGF. This kit exhibits no detectable cross-reactivity with human; TGF-beta, GM-CSF, MCP-3, INF-r, EGF, IL- 1alpha, IL-8, IL-1beta, TNF-alpha, IL-16, MCSF, IL-5, and IL-16.
其它信息1 :
Samples: Serum, plasma, Cell Culture Supernatant, urine, and other biological fluids. Assay Type: Sandwich. Sensitivity: The minimum detectable dose of bFGF was determined by adding two standard deviations to the mean optical density value of the 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 80 pg/mL and using Calibrator Diluent II is 78 pg/mL.
其他信息2 :
Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by using 10 replicates in 1 assay. Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by using replicates on different assays. Intended Uses: This Human bFGF ELISA kit is to be used for the in vitro quantitative determination of human basic fibroblast growth factor (bFGF) concentrations in serum, plasma, cell culture supernatant, urine, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
产品种类 :
ELISA Kits for Human Cytokines and Receptors
产品描述 :
Principle of the assay: This bFGF enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been precoated with a monoclonal specific for bFGF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. bFGF, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound bFGF and other components of sample. In order to quantitative the amount of bFGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for bFGF is added to each well to "sandwich" the bFGF immobilised during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain bFGF and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured by spectrophotometer at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of bFGF in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2- fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus bFGF concentration (pg/mL). The concentration of bFGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI GI登录号 :
8250666
NCBI登录号 :
AAB21432.2
NCBI分子量 :
21,203 Da
NCBI信号通路 :
Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cardiac Progenitor Differentiation Pathway (712094); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Developmental Biology Pathway (477129); Disease Pathway (530764)
NCBI总结 :
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation. Protein type: Motility/polarity/chemotaxis; Activator. Chromosomal Location of Human Ortholog: 4q26. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; growth factor activity; ligand-dependent nuclear receptor transcription coactivator activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; somatic stem cell maintenance; activation of MAPK activity; positive regulation of transcription, DNA-dependent; signal transduction; chemotaxis; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; Ras protein signal transduction; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; inositol phosphate biosynthetic process
尺寸1 :
96 Wells
价格1 :
580美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。