抗体

Mouse Anti-Human TNF alpha-Monocloanl Antibody Datasheet

MBS592180

0.5毫克

465美元

单克隆

小鼠

未共轭

B1E4

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

Mouse Anti-Human TNF alpha-Monocloanl Antibody Datasheet

肿瘤坏死因子-a

肿瘤坏死因子α(肿瘤坏死因子甲);肿瘤坏死因子α(肿瘤坏死因子-a);小鼠单克隆抗体人类肿瘤坏死因子α(肿瘤坏死因子甲)

TNF-alpha; Tumor necrosis factor; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

肿瘤坏死因子-a

肿瘤坏死因子;肿瘤坏死因子;DIF;TNFA;TNFSF2;肿瘤坏死因子甲

TNFA_HUMAN

单克隆

IgG1, k

B1E4

小鼠

Human. Other species not tested

233

这抗体识别重组人类肿瘤坏死因子甲在酶联免疫吸附测定和免疫印迹。 肿瘤坏死因子α(肿瘤坏死因子甲)

蛋白质G亲和纯化

冻干来自a溶液在0.01M PBS, pH值7.0

Store at -20 degree C

酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹)

ELISA: When clone B1E4 is used as detection antibody and clone CH8820 used as capture antibody in an ELISA assay that uses WHO reference material, the detection limit of the ELISA can reach 2.4pg/mL TNF-alpha.

Target Protein: Human TNF-alpha. Immunogen: Purified recombinant human TNF-alpha. Myeloma: Sp2/0-Ag14. Light Chain: Kappa. Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Research Area: Cytokine, inflammation. Product Type: Primary antibody, detection antibody in match antibody pair.

Primary antibodies, Detection antibody in matched antibody pair.; Monoclonal Antibodies to Human Cytokines and Growth Factors

Tumor Necrosis Factor-alpha (TNF-?) is a multifunctional pro-inflammatory cytokine, mainly secreted by activated macrophages. It is implicated with a variety of biological procedures including systemic inflammation, cell proliferation, apoptosis, lipid metabolism, and coagulation. The pleiotropic attribute of TNF-? regulation is associated with its ability to trigger multiple signalling pathways through its receptors, TNFR1 (p55) and TNFR2 (p75). TNF-? not only contributes to the immune response to bacterial, fungal, viral and parasitic invasions, but also functions in tissue remodeling, autoimmune-diseases and the necrosis of specific tumors. TNF-? hyper-expression in response to some bacterial components such as LPS can cause life threatening septic shock. Recombinant TNF-?, in combination with chemotherapy, has been applied for treatment of soft sarcomas, melanomas and other irresectable tumors. Anti-TNF-? therapy has been used for treatment of rheumatoid arthritis.

37210

CAA26669.1

P01375

25,644 Da

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); AhR Pathway (755436); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83097)

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family. Protein type: Membrane protein, integral; Cytokine; Motility/polarity/chemotaxis; Apoptosis. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: extracellular space; recycling endosome; cell surface; integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane; lipid raft; phagocytic cup. Molecular Function: identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding. Biological Process: positive regulation of JNK activity; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of apoptosis; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of caspase activity; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; regulation of immunoglobulin secretion; embryonic gut development; negative regulation of protein complex disassembly; response to drug; positive regulation of cytokine production; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; response to virus; glucose metabolic process; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; negative regulation of fat cell differentiation; positive regulation of chemokine production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; cell activation; detection of mechanical stimulus involved in sensory perception of pain; defense response to Gram-positive bacterium; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of L-glutamate transport; response to activity; negative regulation of transcription, DNA-dependent; skeletal muscle contraction; sequestering of triacylglycerol; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; chronic inflammatory response to antigenic stimulus; response to salt stress; positive regulation of synaptic transmission; positive regulation of hair follicle development; negative regulation of cell proliferation; response to radiation; negative regulation of lipid catabolic process; positive regulation of neuron apoptosis; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of chronic inflammatory response to antigenic stimulus; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; transformed cell apoptosis; calcium-mediated signaling; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; humoral immune response; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; positive regulation of programmed cell death; negative regulation of glucose import; positive regulation of protein complex assembly; positive regulation of chemokine biosynthetic process; negative regulation of viral genome replication; protein import into nucleus, translocation; positive regulation of protein kinase activity; response to hypoxia; activation of MAPKKK activity; positive regulation of fever; positive regulation of protein amino acid phosphorylation; receptor biosynthetic process; negative regulation of myoblast differentiation; leukocyte tethering or rolling; regulation of insulin secretion; positive regulation of cytokine secretion. Disease: Asthma, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1; Malaria, Susceptibility To

0.5毫克

465美元