抗体

a肌动蛋白, 骨骼肌

MBS602996

0.5毫升

450美元

单克隆

小鼠

未共轭

0.N。 4

人类, 大鼠, 鸡, 牛

免疫组化, 免疫细胞化学

抗体

a肌动蛋白, 骨骼肌

a肌动蛋白, 骨骼肌

Anti -a-Actin, Skeletal Muscle

肌动蛋白, 甲骨骼肌;肌动蛋白, 甲骨骼肌;肌动蛋白, 甲骨骼肌;a肌动蛋白;阿尔法肌动蛋白;肌动蛋白, 甲1, 骨骼肌;阿尔法肌动蛋白

ACTA1;ACTA1;ACTA

ACTS_CHICK

单克隆

IgM, k

0.N。 4

小鼠

Bovine, Frog, Guinea Pig, Human, Rabbit, Rat, Sheep

Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i.e. rhabdomyosarcomas). Shows no crossreaction with smooth muscle actin. Species Crossreactivity: bovine, rabbit, sheep, rat, guinea pig and frog.

Purified. Purified by ammonium sulfate precipitation.

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See A0760-24X.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

免疫组化(免疫组化), 免疫荧光(IF)

Suitable for use in Immunofluorescence and Immunohistochemistry. Dilution: Immunohistochemistry (formalin/paraffin sections): 1:200 for 20 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.

Immunogen: N-terminal decapeptide of alpha skeletal muscle isoform of actin; acetylated at the N-terminus. Cellular Localization: Cytoplasmic. Epitope: N-terminal decapeptide.

Positive Control: Skeletal or Cardiac muscle, or Rhabdomyosarcoma

Antibodies; Abs to Actin Related Proteins

71894831

NP_001026234.1

NM_001031063.1

P68139

42,051 Da

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Subcellular location: Cytoplasm cytoskeleton. Post-translational modification: Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization . By similarity. Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Sequence similarities: Belongs to the actin family.

0.5毫升

450美元