抗体

alpha-Gal Epitope (Galalpha1-3Galbeta1-4GlcNAc-R)

MBS603355

1 Vial

625美元

单克隆

小鼠

未共轭

6D407

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪, 流式细胞仪, 酶免疫法

抗体

alpha-Gal Epitope (Galalpha1-3Galbeta1-4GlcNAc-R)

alpha-Gal Epitope

Anti -alpha-Gal Epitope (Gal 1-3Gal 1-4GlcNAc-R)

单克隆

IgM

6D407

小鼠

小鼠, 猪, 兔, 大鼠

Recognizes synthetic and naturally produced rabbit Gal alpha 1-3 Gal epitopes on glycoproteins and glycolipids. Species Crossreactivity: mouse, rat and porcine. Does not crossreact with beta-Gal glycoproteins or BSA.

Supernatant. Supernatant

Supplied as a liquid.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

酶联免疫吸附测定(EL/EIA), 免疫印迹(免疫印迹), 免疫组化(免疫组化), 流式细胞仪(FC/流式细胞仪)

Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Functional Studies (can be used for cytotoxicity assays for alpha-Gal specific pathways).,. Dilution: ELISA: 1:5. Western Blot: 1:5. Flow Cytometry: 1:5. Immunohistochemistry (Paraffin): 1:5. Note: Do not use non-fat milk for blocking. Many blocking agents from non-human mammal sources will crossreact with the antibody.

Immunogen: Rabbit red blood cell membrane

Antibodies; Abs to Enzymes

Widely described antibody against alpha-galactose residues with broad cross-reactivity among different species. Useful for measuring the alpha-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimics in vivo reactions and can be used for cytotoxicity assays for alpha -Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity. Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-alpha-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to alpha-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR.

1 Vial

625美元