产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
碳酸酐酶IX(CA9)(PE)
目录 :
MBS603443
规格 :
100试验
价格 :
695美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
PE
克隆名称 :
[7H20]
反应物种 :
人类
应用 :
流式细胞仪, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS603443 图像 1
Detection of Carbonic Anhydrase IX/CA9 in U 87 MG Human Cell Line by Flow Cytometry using MBS603443.
产品信息
目录号 :
MBS603443
产品类型 :
抗体
产品全称 :
碳酸酐酶IX(CA9)(PE)
产品简称 :
[碳酸酐酶IX]
产品名称同义词 :
[Anti -Carbonic Anhydrase IX (CA9) (PE)]
其他名称 :
[carbonic anhydrase IX; Carbonic anhydrase 9; carbonic anhydrase 9; pMW1; CA-IX; P54/58N; OTTHUMP00000022773; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250; carbonic anhydrase IX; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1]
其他基因名称 :
[CA9;CA9;MN;CAIX;G250;MN]
UniProt数据库进入名 :
CAH9_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG2a
克隆 :
[7H20]
宿主 :
小鼠
反应物种 :
人类
特异性 :
Recognizes human Carbonic Anhydrase IX (CA9). Does not crossreact with recombinant mouse CA9 or with recombinant human CA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
纯度 :
Affinity Purified. Purified by Protein A/G affinity chromatography.
形式 :
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
浓度 :
0.025毫克/毫升
储存稳定性 :
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
检测过的应用 :
流式细胞仪(FC/流式细胞仪)
应用笔记 :
Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: Neat. 10ul labels 1x10e5 cells. Tested using U-87 MG human glioblastoma/astrocytoma cell line. Optimal dilutions to be dertermined by the researcher.
image1头 :
流式细胞仪(FC/流式细胞仪)
其它信息1 :
Immunogen: Recombinant corresponding to aa59-414 of human Carbonic Anhydrase IX (CA9) expressed in NSO cells (Q16790).
其他信息2 :
Flow Cytometry Protocol: Reagents Not Provided: . -PBS (Dulbecco's PBS) -BSA . Intended Use: . Designed to quantitatively determine the percentage of cells bearing Carbonic Anhydrase IX (CA9) within a population and qualitatively determine the density of CA9 on cell surfaces by flow cytometry. Principle of the Test: . Washed cells are incubated with MBS603443, which binds to cells expressing CA9. Unbound phycoerythrin-conjugated antibody is then washed from the cells. Cells expressing CA9 are fluorescently stained: with the intensity of staining directly proportional to the density of expression of CA9. Cell surface expression of CA9 is determined by flow cytometric analysis using 488nm wavelength laser excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 565-605nm. Sample Preparation: . Peripheral Blood Cells:. Whole blood should be collected in evacuated tubes containing EDTA or heparin as the anticoagulant. Contaminating serum components should be removed by washing the cells three times in an isotonic phosphate buffer (supplemented With 0.50/0 BSA) by centrifugation at 500 x g for 5 minutes. Transfer 5Oul of packed cells to a 5ml tube for staining with the monoclonal antibody. Whole blood will require lysis of RBC following the staining procedure. Cell Cultures: Continuous cell lines or activated cell cultures should be centrifuged at 500 x g for 5 minutes and washed three times in an isotonic PBS buffer (supplemented with 0.50/0 BSA), as described above, to remove any residual growth factors that may be present in the culture medium. Cells should then be resuspended in the same buffer to a final concentration of 4xl Oe6 cells/ml and 25ul of cells (1 xl Oe5) transferred to a Sml tube for staining. Note: Adherent cell lines may require pretreatment with O.5mM EDTA to facilitate removal from substrate. Cells that require trypsinization to enable removal from substrate should be further incubated in medium for 6-10 hours on a rocker platform to enable regeneration of the receptors. The use of the rocker platform will prevent reattachment to the substrate. Sample Staining: . 1. Cells should be Fe-blocked by treatment with 1 ug of human IgG/ lOe5 cells for 15 minutes at RT prior to staining. Do not wash excess blocking IgG from this reaction. 2. Transfer 25ul of the Fe-blocked cells (1 xl Oe5 cells) or SOul of packed whole blood to a 5ml tube. 3. Add 1 Oul of Cll 05-80A. Incubate for 30-45 minutes at 4°C. 4. Following this incubation, remove unreacted CA9 reagent by washing the cells twice in 4ml of the same PBS buffer (note: whole blood will require an RBC lysis step at this point using any commercially available lysing reagent). 5. Finally, resuspend the cells in 200-400ul of PBS buffer for final flow cytometric analysis. 6. As a control for analysis, cells in a separate tube should be treated with PE-Iabeled mouse IgG2a antibody. Optimai conditions should be determined by each laboratory.
产品种类 :
Antibodies; Abs to Enzymes
产品描述 :
Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC.
NCBI GI登录号 :
119578763
UniProt数据库登录号 :
Q16790
NCBI信号通路 :
HIF-1-alpha Transcription Factor Network Pathway (138045); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416)
NCBI总结 :
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]
UniProt数据库总结 :
CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. Protein type: Membrane protein, integral; Energy Metabolism - nitrogen; Lyase; Nucleolus; EC 4.2.1.1. Chromosomal Location of Human Ortholog: 9p13.3. Cellular Component: basolateral plasma membrane; microvillus membrane; nucleolus; plasma membrane; integral to membrane. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: response to drug; secretion; bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to testosterone stimulus
尺寸1 :
100试验
价格1 :
695美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。