RhoGAP, p190B (Rho GTPase-activating protein 5) | MyBioSource MBS610564 产品信息

产品简要

公司名称 :

MyBioSource

产品类型 :

抗体

产品名称 :

RhoGAP, p190B (Rho GTPase-activating protein 5)

MBS610564

规格 :

0.1毫升

价格 :

550美元

克隆性 :

多克隆

宿主 :

兔

共轭标签 :

未共轭

反应物种 :

人类, 小鼠, 大鼠, 牛

应用 :

免疫印迹

更多信息或购买 :

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图像

图像 1 :

Western blot anlaysis of extracts from MCF-7 (human), C2C12 (mouse), and BAEC (bovine) cells, using MBS610564.

产品信息

目录号 :

MBS610564

产品类型 :

抗体

产品全称 :

RhoGAP, p190B (Rho GTPase-activating protein 5)

产品简称 :

[RhoGAP, p190B]

产品名称同义词 :

[Anti -RhoGAP, p190B (Rho GTPase-activating protein 5)]

其他名称 :

[rho GTPase-activating protein 5; Rho GTPase-activating protein 5; rho GTPase-activating protein 5; rho-type GTPase-activating protein 5; Rho GTPase activating protein 5; Rho-type GTPase-activating protein 5; p190-B]

其他基因名称 :

[Arhgap5;Arhgap5;p190B;p190-B;Rhogap5]

UniProt数据库进入名 :

RHG05_MOUSE

克隆性 :

多克隆

抗体亚型 :

IgG

宿主 :

兔

反应物种 :

Bovine, Human, Monkey, Mouse, Rat

特异性 :

Recognizes endogneous levels of total human RhoGAP protein at 190kD. Species Crossreactivity: monkey, mouse, rat, bovine.

纯度 :

Affinity Purified. Purified by protein A and peptide affinity chromatography.

形式 :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

储存稳定性 :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

检测过的应用 :

免疫印迹(免疫印迹)

应用笔记 :

Suitable for use in Western Blot. Dilution: Western Blot: 1:1000

image1头 :

免疫印迹(免疫印迹)

其它信息1 :

Immunogen: Synthetic peptide corresponding to a region surrounding Lys296 of human p190-B RhoGAP (KLH).

产品种类 :

Antibodies; Abs to GTPase, Rab, Ras, Rho Proteins

产品描述 :

The Rho family of GTPases, including Rho, Rac and Cdc42, are key regulators of diverse biological processes such as cytoskelatal organization, cell growth and differentiation, transciptional regulation, and cell adhesion/motility (1,2). The activities of these proteins are controlled by GTP binding such that in the GTP-bound state they are active, and in the GDP-bound state they become inactive. Three classes of regulatory proteins control the activity of the GTPases by balancing the levels of GTP/GDP binding: the guanine nucleotide exchange factors (GEFs), which promote the active state by facilitating the exchange of GDP for GTP; the guanine nucleotide dissociation inhibitors GDIs), which sequester the GDP-bound forms and may regulate their intracellular localization; and the GTPase activating proteins (GAPs), which increase GTP hydrolysis to promote the inactive state. The p190 RhoGAP familyconsists of two related proteins, p190-A and p190-B, which are both widely expressed and contain an amino terminal GTPase domain and a carboxy-terminal RhoGAP catalytic domain. Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB (3. Phenotypes of these mice are similar to those of CREB knockouts, with reduced cell size, as well as defects in thymus and neural development (3). Cells deficient in p190-B are also defective for adipogenesis, suggesting this pathway is critical for the adipogenesis-myogeneis switch." (4). There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain (4-6). Levels of tyrosine phosphorylation are enhanced by Src overexpression (5,6). IGF-1 treament downregulates Rho through phosphorylation and activation of p190-BRhoGAP, thereby enhancing IGF signaling implicated in adipogenesis (4).

NCBI GI登录号 :

86262151

NCBI登录号 :

NP_033836.2

NCBI基因登录号 :

NM_009706.2

UniProt数据库登录号 :

P97393

NCBI分子量 :

172,113 Da

NCBI信号通路 :

Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Leukocyte Transendothelial Migration Pathway (83280); Leukocyte Transendothelial Migration Pathway (494); Rho GTPase Cycle Pathway (819819); Signal Transduction Pathway (819739); Signaling By Rho GTPases Pathway (819818)

UniProt数据库总结 :

ARHGAP5: a GTPase activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. Negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Protein type: GAPs, Rac/Rho; Motility/polarity/chemotaxis; GAPs. Biological Process: mammary gland development; positive regulation of mesenchymal cell proliferation; regulation of cell size; positive regulation of cell migration

尺寸1 :

0.1毫升

价格1 :

550美元

更多信息或购买 :

MyBioSource 产品网页

公司信息