抗体

碳酸酐酶IX(CA9)

MBS616120

0.1毫升

625美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠, 狗

免疫印迹, 免疫细胞化学

抗体

碳酸酐酶IX(CA9)

[碳酸酐酶IX]

[Anti -Carbonic Anhydrase IX (CA9)]

[carbonic anhydrase IX; Carbonic anhydrase 9; carbonic anhydrase 9; pMW1; CA-IX; P54/58N; OTTHUMP00000022773; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250; carbonic anhydrase IX; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1]

[CA9;CA9;MN;CAIX;G250;MN]

CAH9_HUMAN

多克隆

IgG

兔

狗, 小鼠和大鼠

识别人类碳酸酐酶IX

亲和纯化。 经免疫亲和层析纯化

以溶解在PBS液体方式提供, pH值7.5, 0.025% 叠氮化钠

1.0毫克/毫升

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

适合用途包括免疫荧光/免疫细胞化学, 免疫印迹, 流式细胞仪和免疫组化。 其他未被检测的应用

Immunofluorescence (IC): 1:1000 . Western Blot: 1:2000. Flow Cytometry: 1:1000. Immunohistochemistry (Frozen, paraffin): 1:1000. Optimal dilutions to be determined by the researcher

试验数据

免疫组化(免疫组化)

免疫组化(免疫组化)

免疫荧光(IF)

Immunofluorescence of HEK 293 cells using MBS616120. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.

Immunogen: Synthetic peptide corresponding to a C-terminal sequence of the human carbonic anhydrase IX. Cellular Localization: Cell Membranes

Positive Control: Renal carcinoma

Antibodies; Abs to Enzymes

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

119578763

Q16790

HIF-1-alpha Transcription Factor Network Pathway (138045); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. Protein type: Membrane protein, integral; Nucleolus; Energy Metabolism - nitrogen; Lyase; EC 4.2.1.1. Chromosomal Location of Human Ortholog: 9p13.3. Cellular Component: basolateral plasma membrane; microvillus membrane; nucleolus; integral to membrane; plasma membrane. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: response to drug; secretion; bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to testosterone stimulus

0.1毫升

625美元