抗体

Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)

MBS619087

0.1毫克

730美元

多克隆

羊

未共轭

人类, 小鼠

流式细胞仪, 酶免疫法

抗体

Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)

[Indoleamine 2,3-Dioxygenase]

[Sheep anti-Human Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)]

[indoleamine 2,3-dioxygenase; Indoleamine 2,3-dioxygenase 1; indoleamine 2,3-dioxygenase 1; IDO-1; OTTMUSP00000022551; OTTMUSP00000022552; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1; Indoleamine-pyrrole 2,3-dioxygenase]

[Ido1; Ido1; Ido; Indo; Ido; Indo]

I23O1_MOUSE

多克隆

IgG

羊

人类

Recognizes human indoleamine 2,3-dioxygenase (IDO). Reactivity with IDO has been confirmed on Western Blot with IFN-gamma stimulated human peripheral blood lymphocytes.

纯化

以溶解在PBS液体方式提供, 0.1% BSA, 0.02% 叠氮化钠

0.1毫克/毫升

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

酶联免疫吸附测定(EL/EIA), 免疫印迹(免疫印迹), 免疫组化(免疫组化), 流式细胞仪(FC/流式细胞仪), 免疫荧光(IF)。 其他未被检测的应用

Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:50; Detection antibody. Immunofluorescence: 1:50. Flow Cytometry: 1:50; It's recommended that this antibody be used in combination with fixing and permeabilization. Western Blot: 1:50. Immunohistochemistry (Paraffin): 1:50. Optimal dilutions to be determined by the researcher

Immunogen: Recombinant human IDO

Antibodies; Abs to Enzymes, Oxidase

Human indoleamine 2,3-dioxygenase (IDO) is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules, like tryptophan, serotonin and melatonin. By doing this, IDO initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO induction. The production of INF-gamma and induction of IDO represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia. By local depletion of tryptophan and increasing proapoptotic kynurenines, IDO greatly affects T-cell proliferation and survival, both in vitro and in vivo, and also affects B-cell and NK-cell function and survival. There is a central role for IDO expression in tolerance involving regulatory cells and DCs. IDO acts as an intermediate pathway in LPS-induced production of reactive oxygen species and NF-kappaB activation, two processes that lead to DC maturation.

194047

P28776

45,641 Da

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Metabolic Pathways (132962); Metabolism Of Amino Acids And Derivatives Pathway (367087); Tryptophan Catabolism Pathway (367094); Tryptophan Metabolism Pathway (83163); Tryptophan Metabolism Pathway (198332); Tryptophan Metabolism Pathway (332)

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family. Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.13.11.52; Cell cycle regulation. Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytoplasm; cytosol. Molecular Function: amino acid binding; protein binding; dioxygenase activity; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; oxidoreductase activity; oxygen binding; tryptophan 2,3-dioxygenase activity. Biological Process: positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; positive regulation of apoptosis; negative regulation of activated T cell proliferation; response to lipopolysaccharide; defense response; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; inflammatory response; tryptophan catabolic process; multicellular organismal response to stress; positive regulation of T cell tolerance induction

0.1毫克

730美元