抗体

Tankyrase 2 (TNKS2, Tankyrase, TRF1-interacting Ankyrin-related ADP-ribose polymerase 2, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, Tankyrase 2)

MBS621201

0.1毫克

490美元

多克隆

山羊

未共轭

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

Tankyrase 2 (TNKS2, Tankyrase, TRF1-interacting Ankyrin-related ADP-ribose polymerase 2, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, Tankyrase 2)

端锚聚合酶类2

Anti -Tankyrase 2 (TNKS2, Tankyrase, TRF1-interacting Ankyrin-related ADP-ribose polymerase 2, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, Tankyrase 2)

tankyrase-2; Tankyrase-2; tankyrase-2; TNKS-2; tankyrase 2; tankyrase II; OTTHUMP00000020090; tankyrase-like protein; tankyrase-related protein; poly [ADP-ribose] polymerase 5B; TRF1-interacting ankyrin-related ADP-ribose polymerase 2; tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2; Poly [ADP-ribose] polymerase 5B; TNKS-2; TRF1-interacting ankyrin-related ADP-ribose polymerase 2; Tankyrase II; Tankyrase-like protein; Tankyrase-related protein

TNKS2;TNKS2;TNKL;TANK2;pART6;PARP5B;PARP5C;PARP-5b;PARP-5c;PARP5B;TANK2;TNKL

TNKS2_HUMAN

多克隆

山羊

人类

Recognizes human Tankyrase 2 at ~125kD. Species Sequence Homology: mouse and canine

Affinity Purified. Purified by affinity chromatography.

Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

酶联免疫吸附测定(EL/EIA), 免疫印迹(免疫印迹)

Suitable for use in Peptide ELISA and Western Blot. Dilution: Peptide ELISA: 1:16,000. Western Blot: 0.5-1.5ug/ml. Observed band at ~125kD. An additional band of unknown identity was observed at ~45kD.

Immunogen: Synthetic peptide with sequence C-HRRKEVSEENHNH, from the internal region of human Tankyrase 2 (NP_079511.1).

Positive Control: Jurkat cell line lysates

Antibodies; Abs to Enzymes, Polymerase

Tankyrase (TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyase 1, TANK1, TNKS1) is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (1). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase co-localizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes. Tankyrase 2 (TRF1-interacting akyrin-related ADP-ribose polymerase 2, TANK2, TNKS2) is a closely related homolog of tankyrase 1. Tankyrase 2 lacks the N-terminus HPS domain (containing homo-polymeric runs of histidine, proline, and serine) found in tankyrase 1.

13376842

NP_079511.1

NM_025235.3

Q9H2K2

126,918 Da

TNKS2: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP- ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Protein type: EC 2.4.2.30; Transferase; Nuclear envelope. Chromosomal Location of Human Ortholog: 10q23.3. Cellular Component: Golgi membrane; pericentriolar material; nuclear chromosome, telomeric region; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; cytosol; nucleus. Molecular Function: protein binding; enzyme binding; metal ion binding; NAD+ ADP-ribosyltransferase activity. Biological Process: protein amino acid ADP-ribosylation; Wnt receptor signaling pathway; protein polyubiquitination; multicellular organism growth; regulation of multicellular organism growth; positive regulation of telomere maintenance via telomerase

0.1毫克

490美元