抗体

血管内皮生长因子受体2, 磷酸化(Tyr951)(血管内皮生长因子R2, Flk1, 激酶插入区受体, 受体酪氨酸激酶)

MBS631534

0.1毫升

600美元

单克隆

兔

未共轭

[5i133]

人类, 小鼠

抗体

血管内皮生长因子受体2, 磷酸化(Tyr951)(血管内皮生长因子R2, Flk1, 激酶插入区受体, 受体酪氨酸激酶)

[血管内皮生长因子受体2]

[Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)]

[vascular endothelial growth factor receptor 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor 2; soluble VEGFR2; OTTHUMP00000158928; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1]

[激酶插入区受体;激酶插入区受体;FLK1;CD309;VEGFR;VEGFR2;FLK1]

VGFR2_HUMAN

单克隆

IgG

[5i133]

兔

人类, 小鼠

Recognizes human VEGF receptor 2 only when phosphorylated at Tyr951 at ~230kD. May crossreact with activated VEGF receptor 1, but does not react with other related tyrosine phosphorylated tyrosine kinases. Species Crossreactivity: mouse

纯化

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

适合用途包括酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹), 和免疫组化(免疫组化)。 其他未被检测的应用

Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking , overnigth. Immunohistochemistry(Paraffin): 1:125 Requires HIER using EDTA, pH 8.0 prior to staining and TBST-5% NGS as antibody diluent. Optimal dilutions to be determined by the researcher.

免疫印迹(免疫印迹)

免疫印迹(免疫印迹)

免疫组化(免疫组化)

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr951 of human VEGF receptor 2 (KLH). Grade: Purified

Antibodies; Abs to Receptors

Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2. The phosphorylation of Tyr1212 provides a docking site for Grb2 binding, and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCg, as well as Shb. Signaling from VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo.

11321597

NP_002244.1

NM_002253.2

P35968

Angiogenesis Pathway (198772); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); HIF-2-alpha Transcription Factor Network Pathway (137956); Id Signaling Pathway (198871)

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq]

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability. Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; VEGFR family. Chromosomal Location of Human Ortholog: 4q11-q12. Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; plasma membrane; cell junction; nucleus; endosome; lipid raft; external side of plasma membrane. Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; protein-tyrosine kinase activity; growth factor binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; Hsp90 protein binding; ATP binding. Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; positive regulation of focal adhesion formation; positive regulation of MAPKKK cascade; ovarian follicle development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; lymph vessel development; alveolus development; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of apoptosis. Disease: Hemangioma, Capillary Infantile

0.1毫升

600美元