抗体

抗人类CD38-异硫氰酸荧光素

MBS666491

120试验

310美元

单克隆

小鼠

异硫氰酸荧光素

[AT1]

流式细胞仪, 流式细胞仪

抗体

抗人类CD38-异硫氰酸荧光素

[CD38]

[单克隆抗人类CD38/生物素]

[CD38; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; CD38 antigen (p45); NAD(+) nucleosidase; ADP-ribosyl cyclase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; CD38 molecule; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20); 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD_antigen: CD38]

[CD38]

[CD38;CD38;T10;ADPRC 1;ADPRC 1;环磷酸腺苷核糖水解酶1]

CD38_HUMAN

单克隆

IgG1

[AT1]

小鼠

Human, Cynomolgus

300

1.0毫克/毫升

Store at 2 - 5°C. Freeze/thawing not recommended. Protect from light. Product should retain activity for at least 12 months after shipping date when stored as recommended.

流式细胞仪(FC/流式细胞仪)

Working dilution : 1:50 (or use 1.6 uL of concentrated stock per 5 x 10 5 -cell test)

试验数据

Performance: Five x 10 5 cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD38/Biotin at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.94 log 10 fluorescent units when compared to a Mouse IgG1/Biotin negative control (MBS666714) at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD38 antibody (MBS666469). Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN 3 (as a preservative).

Conjugation: FITC . Production: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.25 mg/mL with a Fluorescein/IgG molar ratio of 7.1.

Information: Human CD38 is a type II transmembrane glycoprotein with bifunctional ectoenzyme activity catalyzing both synthesis and hydrolysis of cyclic ADP ribose (1). CD38 is involved in lymphocyte activation and adhesion to endothelium and has recently been identified as a counter-receptor of CD31 (2). Antibody AT1 recognizes the CD38 molecule of 45 kd.

1.) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 151-157. 2.) S. Deaglio, et al, (1998) J Immunol 160:395-402.

1911103

BAA18966.1

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Metabolic Pathways (132956); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Oxytocin Signaling Pathway (952859)

CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq, Jul 2008]

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.2.2.6; Membrane protein, integral; Lyase; Apoptosis; EC 2.4.99.20; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cell cycle regulation; Hydrolase; Cell surface. Chromosomal Location of Human Ortholog: 4p15. Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; nucleus. Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity. Biological Process: response to drug; response to retinoic acid; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; female pregnancy; signal transduction; positive regulation of cell growth; response to estradiol stimulus; elevation of cytosolic calcium ion concentration; B cell receptor signaling pathway; response to hydroperoxide; positive regulation of B cell proliferation; response to hypoxia; positive regulation of vasoconstriction; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; negative regulation of bone resorption; negative regulation of apoptosis

120试验

310美元