抗体

CD33 单克隆抗体

MBS668002

0.1毫克

175美元

单克隆

小鼠

未共轭

[ABM29D3]

免疫印迹, 免疫组化, 流式细胞仪

抗体

CD33 单克隆抗体

[CD33 ]

[myeloid cell surface antigen CD33 isoform 2; Myeloid cell surface antigen CD33; myeloid cell surface antigen CD33; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; CD33 molecule; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33]

[CD33 ]

[CD33; CD33; p67; SIGLEC3; SIGLEC-3; SIGLEC3; Siglec-3]

CD33_HUMAN

单克隆

Mouse IgG2a, Kappa

[ABM29D3]

小鼠

人类

237

纯化(液体)

0.5毫克/毫升, 200微升

Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.

Western Blot (WB), Immunohistochemistry (IHC), Fluorescence-activated cell sorting (FACS)

Western blot analysis: 2-4 ug/ml. Immunohistochemical analysis: 5 ug/ml. FACS: 0.2-0.5 ug/10 6 cells

免疫印迹(免疫印迹)

免疫组化(免疫组化)

免疫组化(免疫组化)(免疫组化)

免疫组化(免疫组化)

Immunohistochemical analysis of CD33  in squamous cell carcinoma of esophagus using CD33 antibody (Clone: ABM29D3) at 5 ug/ml.

流式细胞仪

Cell Surface flow analysis of hCD33 in PBMC (Monocytes) using 0.2?g/10^6 cells of CD33 clone (ABM29D3). Green represents isotype control; red represents antihCD33 antibody. Goat anti-mouse PE conjugated secondary antibody was used.

Antigen: CD33

Immunogen: A partial recombinant CD33 protein (amino acids 28-320) was used as the immunogen for this antibody.

免疫学

CD33 is a member of the SIGLEC (Sialic Acid-Binding Ig-Like Lectin) family of receptors, and the gene comprises seven coding exons. Exon 2 encodes the canonical IgV domain, exon 4 encodes the IgC structural domain, and exons 6 and 7 encode cytosolic ITIMs (Immunotyrosine Inhibitory Motifs). CD33 acts as a cell surface antigen which is expressed on normal myeloid cells and CD34+ blasts in AML (Acute Myeloid Leukemia). The antigen serves as a target of GO (Gemtuzumab/Ozogamicin), which exerts anti-leukemic effects in refractory AML.

130980072

NP_001076087.1

NM_001082618.1

P20138

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: protein binding; receptor activity; carbohydrate binding. Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion

0.1毫克

175美元