产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
大鼠抗小鼠PDGFR乙
目录 :
MBS690560
规格 :
0.1毫克
价格 :
475美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
(4C54)
反应物种 :
小鼠
应用 :
免疫印迹, 免疫组化, 免疫组化-石蜡切片
更多信息或购买 :
产品信息
目录号 :
MBS690560
产品类型 :
抗体
产品全称 :
大鼠抗小鼠PDGFR乙
产品简称 :
PDGFR乙
产品名称同义词 :
抗小鼠血小板衍生生长因子受体乙
其他名称 :
platelet-derived growth factor receptor beta isoform 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; PDGF beta chain; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; platelet-derived growth factor receptor beta variant 1; platelet derived growth factor receptor, beta polypeptide; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1
产品基因名称 :
PDGFR乙
其他基因名称 :
Pdgfrb; Pdgfrb; Pdgfr; CD140b; PDGFR-1; AI528809; Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1
UniProt数据库进入名 :
PGFRB_MOUSE
克隆性 :
单克隆
抗体亚型 :
IgG2
克隆 :
(4C54)
宿主 :
大鼠
反应物种 :
小鼠
序列长度 :
1099
纯度 :
蛋白质G亲和色谱
形式 :
冻干
储存稳定性 :
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Shipping: Ships on cold packs
检测过的应用 :
免疫印迹(免疫印迹), 免疫组化(免疫组化)-石蜡
应用笔记 :
1. Western blot: Yes (1:500-1000). 2. Immunohistochemistry (Paraffin): Yes (1:50-200)
产品描述 :
Background/Introduction: The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, B, C, and D) that form disulfidelinked homodimers (PDGF-AA, BB, CC, and DD) and a heterodimer (PDGF-AB). These proteins regulate diverse cellular functions by binding to and inducing the homo- or heterodimerization of two receptors (PDGF Ralpha and Rbeta). Whereas alpha/alpha homodimerization is induced by PDGF-AA, BB, CC, and AB, alpha/beta heterodimerization is induced by PDGF-AB, -BB, CC, and DD, and beta/beta homodimerization is induced only by PDGF-BB, and DD. Both PDGF-Ralpha and -Rbeta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF Ralpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG63. Both the recombinant mouse and human soluble PDGF Ralpha bind PDGF with high affinity and are potent PDGF antagonists.
NCBI GI登录号 :
226342982
NCBI登录号 :
NP_001139740.1
NCBI基因登录号 :
NM_001146268.1
UniProt数据库登录号 :
P05622
NCBI分子量 :
122,806 Da
NCBI信号通路 :
Adaptive Immune System Pathway (926013); Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Constitutive PI3K/AKT Signaling In Cancer Pathway (926316); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (926127); DAP12 Signaling Pathway (926128); Disease Pathway (926229); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (926027)
UniProt数据库总结 :
Function: Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca2+ and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM . By similarity. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor. Ref.2 Ref.6 Ref.8 Ref.10 Ref.12 Ref.13 Ref.15 Ref.17 Ref.18. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.8. Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. Binding of PDGFB and/or PDGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Subunit structure: Interacts with homodimeric PDGFB and PDGFD, and with heterodimers formed by PDGFA and PDGFB. May also interact with homodimeric PDGFC. Monomer in the absence of bound ligand. Interaction with homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD, leads to receptor dimerization, where both PDGFRA homodimers and heterodimers with PDGFRB are observed. Interacts with SH2B2/APS. Interacts directly (tyrosine phosphorylated) with SHB. Interacts (tyrosine phosphorylated) with PIK3R1. Interacts (tyrosine phosphorylated) with CBL. Interacts (tyrosine phosphorylated) with SRC and SRC family kinases. Interacts (tyrosine phosphorylated) with PIK3C2B, maybe indirectly. Interacts (tyrosine phosphorylated) with SHC1, GRB7, GRB10 and NCK1. Interaction with GRB2 is mediated by SHC1. Interacts (via C-terminus) with SLC9A3R1 . By similarity. Ref.2 Ref.4 Ref.8 Ref.11 Ref.12 Ref.17 Ref.19. Subcellular location: Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle . By similarity. Lysosome lumen . By similarity. Note: After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation. Tissue specificity: Weakly expressed in glomerular mesangial cells and interstitial cells. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, increased expression in interstitial cells at day 4 and expression is markedly elevated at day 7 and is maximal at day 14. Ref.5. Post-translational modification: Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-578, and to a lesser degree, Tyr-580 is important for interaction with SRC. Phosphorylation at Tyr-715 is important for interaction with GRB2. Phosphorylation at Tyr-739 and Tyr-750 is important for interaction with PIK3R1. Phosphorylation at Tyr-750 is important for interaction with NCK1. Phosphorylation at Tyr-770 and Tyr-856 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-856 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1008 is important for interaction with PTPN11. Phosphorylation at Tyr-1008 and Tyr-1020 is important for interaction with PLCG1. Dephosphorylated by PTPRJ at Tyr-750, Tyr-856, Tyr-1008 and Tyr-1020 . By similarity. Dephosphorylated by PTPN2 at Tyr-578 and Tyr-1020. Ref.2 Ref.9 Ref.10N-glycosylated . By similarity.Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation. Ref.11. Disruption phenotype: No apparent phenotype up to 16 dpc. Lethality late during gestation or at birth, due to widespread bleedings. This is due to a severe shortage of vascular smooth muscle cells and pericytes, especially in the central nervous system, skin, lung and heart. Mutants suffer from hemorrhages, anemia, thrombocytopenia, and show defects in the formation of kidney glomeruli, due to a lack of mesangial cells. Ref.3 Ref.7 Ref.14. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.
尺寸1 :
0.1毫克
价格1 :
475美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。