抗体

仓鼠抗小鼠CD80

MBS690572

0.1毫克

490美元

单克隆

仓鼠

未共轭

[(MAB0713)]

免疫组化, 流式细胞仪, 流式细胞仪

抗体

仓鼠抗小鼠CD80

[CD80]

[Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17]

[T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen; CD80 antigen; Activation B7-1 antigen]

[CD80]

[Cd80; Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17; B7; B7]

CD80_MOUSE

单克隆

IgG

[(MAB0713)]

亚美尼亚仓鼠

小鼠

306

蛋白质G/A色谱

冻干

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

免疫组化(免疫组化), 流式细胞仪(FC/流式细胞仪)

IHC (paraffine): 1:100-1:300. Flow cytometry: 1:100-1:1000

免疫组化(免疫组化)

免疫组化(免疫组化)

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a Armenian Hamster immunized with recombinant Mouse CD80 protein. IgG fraction of the culture supernatant was purified by Protein G affinity chromatography. Reconstitution buffer: PBS(sterile). Antigen: Recombinant Mouse CD80 protein

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Remarks: This antibody was selected for its ability to detect mouse CD80.

B7-1 (CD80) and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the downregulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be upregulated through interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily.

112983684

NP_033985.3

NM_009855.2

Q00609

Adaptive Immune System Pathway (926013); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (926020); CD28 Dependent PI3K/Akt Signaling Pathway (926021); CD28 Dependent Vav1 Pathway (926022); CTLA4 Inhibitory Signaling Pathway (926023); Cell Adhesion Molecules (CAMs) Pathway (83266)

Function: Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Subcellular location: Membrane; Single-pass type I membrane protein. Tissue specificity: Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies. Developmental stage: Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation. Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

0.1毫克

490美元