蛋白

重组小鼠FGF-23

MBS691906

0.005毫克

200美元

重组蛋白

重组小鼠FGF-23

[FGF-23]

[成纤维细胞生长因子23]

[成纤维细胞生长因子23;成纤维细胞生长因子23;成纤维细胞生长因子23;FGF-23;成纤维细胞生长因子23]

[FGF-23]

[Fgf23;Fgf23;FGF-23]

FGF23_MOUSE

小鼠

228

MYPDTSPLLG SNWGSLTHLY TATARTSYHL QIHRDGHVDG TPHQTI YSAL MITSEDAGSV VITGAMTRRF LCMDLHGNIF GSLHFSPENC K FRQWTLENG YDVYLSQKHH YLVSLGRAKR IFQPGTNPPP FSQFLAR RNE VPLLHFYTVR PRRHTRSAED PPERDPLNVL KPRPRATPVP VS CSRELPSA EEGGPAASDP LGVLRRGRGD ARGGAGGADR CRPFPRFV

> 95% by SDS-PAGE & HPLC analyses

冻干

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

Expressed in: E.coli. Biological Activity: Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells. The expected ED 50 for this effect is 0.3-0.5 ug/ml, in the presence of murine Klotho and heparin. Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug)

Reconstitution: Centriguge the vial before opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it recommended to further dilute in a buffer containing a carrier protein (example: 0.1% BSA) and store in working aliquots at -20°C to -80°C.

细胞因子&生长因子

The FGF family plays a central role during prenatal development and postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-23, FGF-21 and FGF-19 constitute an atypical FGF subfamily whose ligands act as circulating hormones and require the participation of a Klotho protein as a co-receptor for their signaling. FGF-23 is a bone-derived hormone that acts in the kidney to regulate phosphate homeostasis and vitamin D metabolism. The signaling receptor for FGF-23, a Klotho-FGFR1 (IIIc) complex, is an essential regulator of the renal sodium phosphate co-transporter and key vitamin D-metabolizing enzymes CYP27B1 and CYP24A1. Recombinant murine FGF-23 is a 25.5 kDa globular protein containing 228 amino acid residues.

12083589

NP_073148.1

NM_022657.4

Q9EPC2

25.5 kDa

Activated Point Mutants Of FGFR2 Pathway (926270); Adaptive Immune System Pathway (926013); Constitutive PI3K/AKT Signaling In Cancer Pathway (926316); DAP12 Interactions Pathway (926127); DAP12 Signaling Pathway (926128); Disease Pathway (926229); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (926027); Downstream Signal Transduction Pathway (926957); Downstream Signaling Of Activated FGFR Pathway (926257); ESC Pluripotency Pathways (198374)

This gene encodes a member of the fibroblast growth factor family. The encoded protein regulates phosphate homeostasis and vitamin D metabolism. Mutation of the related gene in humans causes autosomal dominant hypophosphatemic rickets (ADHR). The secreted protein is further cleaved into N- and C-terminal chains, which results in loss of function. [provided by RefSeq, Mar 2013]

Function: Regulator of phosphate homeostasis . By similarity. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels . By similarity. Acts directly on the parathyroid to decrease PTH secretion . By similarity. Regulator of vitamin-D metabolism . By similarity. Negatively regulates osteoblasts differentiation and matrix mineralization . By similarity. Upregulates EGR1 expression in the presence of KL. Ref.3. Subunit structure: Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL and heparan sulfate glycosaminoglycans that function as coreceptors . By similarity. Ref.3. Subcellular location: Secreted . By similarity. Note: Secretion is dependent on O-glycosylation . By similarity. Tissue specificity: Mainly expressed in the brain and thymus at low levels. In brain; preferentially expressed in the ventrolateral thalamic nucleus. Post-translational modification: Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases . By similarity.O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23 . By similarity. Sequence similarities: Belongs to the heparin-binding growth factors family.

0.005毫克

200美元

0.02毫克

340