ELISA/assay

鱼类白细胞介素1beta(IL- 1beta)酶联免疫吸附试剂盒

MBS700230

48-Strip-Wells

565美元

酶联免疫吸附试剂盒

鱼类白细胞介素1beta(IL- 1beta)酶联免疫吸附试剂盒

白细胞介素1beta(IL- 1beta)

白细胞介素1beta(IL- 1beta);白细胞介素1b(IL- 1b);鱼类白细胞介素1b(IL- 1b)酶联免疫吸附试剂盒

interleukin-1 beta proprotein; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; catabolin; OTTHUMP00000162031; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1, beta; Catabolin

IL- 1beta

IL- 1b

IL1B;IL1B;IL-1;IL1F2;IL1-乙;IL1F2

IL1B_HUMAN

Fish

This assay has high sensitivity and excellent specificity for detection of fish IL-1beta. No significant cross-reactivity or interference between fish IL-1beta and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: 0.78 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL- 1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL- 1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL- 1beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL- 1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

10835145

NP_000567.1

NM_000576.2

P01584

30,748 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076); Chagas Disease (American Trypanosomiasis) Pathway (147809)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]

Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Ref.16. Subunit structure: Monomer. Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. Sequence similarities: Belongs to the IL-1 family.

48-Strip-Wells

565美元

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150