产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
兔抗人类凝溶胶蛋白多克隆抗体(GSN)
目录 :
MBS7003440
规格 :
0.05毫克
价格 :
160美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS7003440 图像 1
Western Blot. Positive WB detected in: THP-1 whole cell lysate. All lanes: GSN antibody at 3.5ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 86, 81, 82 kDa. Observed band size: 86 kDa
图像 2 :
MyBioSource MBS7003440 图像 2
IHC image of MBS7003440 diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica Bond system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
图像 3 :
MyBioSource MBS7003440 图像 3
Immunofluorescence staining of Hela cells with MBS7003440 at 1:100, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
产品信息
目录号 :
MBS7003440
产品类型 :
抗体
产品全称 :
兔抗人类凝溶胶蛋白多克隆抗体(GSN)
产品简称 :
[凝溶胶蛋白]
产品名称同义词 :
[Gelsolin (AGEL) (Actin-depolymerizing factor) (ADF) (Brevin), GSN]
其他名称 :
[gelsolin isoform a; Gelsolin; AGEL; Actin-depolymerizing factor; ADF; Brevin]
其他基因名称 :
[GSN;ADF]
UniProt数据库进入名 :
GELS_HUMAN
克隆性 :
多克隆
抗体亚型 :
IgG
宿主 :
反应物种 :
人类
纯度 :
>95%, Protein G purified
形式 :
液体
储存稳定性 :
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
检测过的应用 :
酶联免疫吸附测定, 免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF)
应用笔记 :
Recommended dilution: WB: 1:500-1:5000, IHC: 1:200-1:500, IF: 1:50-1:200
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其它信息1 :
Immunogen: Recombinant Human Gelsolin protein (28-782AA). Conjugate: Non-conjugated. Storage Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Species: Human. Research Area: Signal Transduction
其他信息2 :
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6405 / sc-6406 / sc-22602 / sc-22600 / sc-48769
产品描述 :
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (endblocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
NCBI GI登录号 :
4504165
NCBI登录号 :
NP_000168.1
NCBI基因登录号 :
NM_000177.4
UniProt数据库登录号 :
P06396
NCBI信号通路 :
Amyloid Fiber Formation Pathway (1269169); Apoptosis Pathway (1270262); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1270289); Coregulation Of Androgen Receptor Activity Pathway (138085); EGF Receptor (ErbB1) Signaling Pathway (137970); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590)
UniProt数据库总结 :
Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Nuclear receptor co-regulator; Actin-binding. Chromosomal Location of Human Ortholog: 9q33. Cellular Component: actin cap; actin cytoskeleton; cortical actin cytoskeleton; cytoplasm; cytosol; extracellular region; extracellular space; focal adhesion; lamellipodium; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; ruffle; sarcoplasm. Molecular Function: actin binding; calcium ion binding; myosin II binding; protein binding; protein domain specific binding. Biological Process: actin filament capping; actin filament polymerization; actin filament severing; actin nucleation; aging; apoptosis; barbed-end actin filament capping; cell structure disassembly during apoptosis; cellular protein metabolic process; extracellular matrix disassembly; negative regulation of virion penetration into host cell; oligodendrocyte development; phagocytosis, engulfment; phosphoinositide-mediated signaling; positive regulation of actin nucleation; programmed cell death; protein destabilization; response to ethanol; response to folic acid; sequestering of actin monomers; striated muscle atrophy; tissue regeneration. Disease: Amyloidosis, Finnish Type
尺寸1 :
0.05毫克
价格1 :
160美元
尺寸2 :
0.1毫克
价格2 :
235
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。