ELISA/assay

Human Complement Fragment 3d, C3d ELISA Kit

MBS700455

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Human Complement Fragment 3d, C3d ELISA Kit

Complement Fragment 3d, C3d

Human Complement Fragment 3d; C3d ELISA Kit; Complement Fragment 3d; C3d

C3d

人类

This assay has high sensitivity and excellent specificity for detection of human C3d. No significant cross-reactivity or interference between human C3d and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 mug/ml -10 mug/ml. Sensitivity: The minimum detectable dose of human C3d is typically less than 0.039 mug/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3d has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3d present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3d is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3d bound in the initial step. The color development is stopped and the intensity of the color is measured.

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800