ELISA/assay

兔肿瘤坏死因子α, TNFalpha酶联免疫吸附试剂盒

MBS700705

48-Strip-Wells

465美元

酶联免疫吸附试剂盒

兔肿瘤坏死因子α, TNFalpha酶联免疫吸附试剂盒

tumor necrosis factor (TNF superfamily, member 2)

Rabbit Tumor necrosis factor alpha; TNFalpha ELISA Kit; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily; member 2)

tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

肿瘤坏死因子

肿瘤坏死因子;肿瘤坏死因子;TNFA;TNFSF2;TNFA;TNFSF2;肿瘤坏死因子-a;NTF;ICD1;ICD2

TNFA_RABIT

兔

234

This assay has high sensitivity and excellent specificity for detection of rabbit TNFalpha. No significant cross-reactivity or interference between rabbit TNFalpha and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 78 pg/ml -5000 pg/ml. Sensitivity: 19.5 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFalpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFalpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFalpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFalpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

126722916

NP_001075732.1

NM_001082263.1

P04924

25,817 Da

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167324)

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

48-Strip-Wells

465美元

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595