ELISA/assay

猪髓过氧化酶, MPO酶联免疫吸附试剂盒

MBS700708

48-Strip-Wells

605美元

酶联免疫吸附试剂盒

猪髓过氧化酶, MPO酶联免疫吸附试剂盒

髓过氧化酶, MPO

猪髓过氧化酶(MPO)酶联免疫吸附试剂盒;髓过氧化酶;MPO

髓过氧化酶;髓过氧化酶;髓过氧化酶;髓过氧化酶

MPO

MPO;MPO;MPO

PERM_HUMAN

猪

745

This assay has high sensitivity and excellent specificity for detection of pig MPO. No significant cross-reactivity or interference between pig MPO and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 ng/ml -1000 ng/ml. Sensitivity: 3.9 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557759

NP_000241.1

NM_000250.1

P05164

87,248 Da

C-MYB Transcription Factor Network Pathway (138073); Folate Metabolism Pathway (198833); IL23-mediated Signaling Events Pathway (138000); Phagosome Pathway (153910); Phagosome Pathway (153859); Selenium Pathway (198825); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Vitamin B12 Metabolism Pathway (920974); Amb2 Integrin Signaling Pathway (137945)

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Catalytic activity: Cl- + H2O2 + H+ = HClO + H2O. Cofactor: Binds 1 calcium ion per monomer.

48-Strip-Wells

605美元

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535