ELISA/assay

人类分泌成分, SC酶联免疫吸附试剂盒

MBS701535

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

人类分泌成分, SC酶联免疫吸附试剂盒

分泌成分, SC

人类分泌成分;SC酶联免疫吸附试剂盒;分泌成分;SC

secretory component; Protein translocase subunit SecY

SC

secY

SECY_STRLI

人类

437

This assay has high sensitivity and excellent specificity for detection of Human SC. No significant cross-reactivity or interference between Human SC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 g/ml-20 g/ml. Sensitivity: 0.078 g/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SC bound in the initial step. The color development is stopped and the intensity of the color is measured.

862997

AAC44590.1

P49977

47,312 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently UniRule annotationHAMAP-Rule:MF_01465

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800