ELISA/assay

人类14-3蛋白乙/甲, YWHAB酶联免疫吸附试剂盒

MBS701844

48-Strip-Wells

565美元

酶联免疫吸附试剂盒

人类14-3蛋白乙/甲, YWHAB酶联免疫吸附试剂盒

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide

Human 14-3-3 protein beta/alpha (YWHAB) ELISA kit; GW128; HS1; KCIP-1; 14-3-3 protein beta/alpha; brain protein 14-3-3; beta isoform; protein 1054; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; beta polypeptide

14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; epididymis secretory protein Li 1; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1

YWHAB

YWHAB;YWHAB;HS1;GW128;YWHAA;KCIP-1;HEL-S-1;KCIP-1

1433B_HUMAN

人类

246

This assay has high sensitivity and excellent specificity for detection of Human YWHAB. No significant cross-reactivity or interference between Human YWHAB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml-400 ng/ml. Sensitivity: 1.56 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAB bound in the initial step. The color development is stopped and the intensity of the color is measured.

4507949

NP_003395.1

NM_003404.4

P31946

28,082 Da

ARMS-mediated Activation Pathway (106466); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adaptive Immune System Pathway (366160); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Axon Guidance Pathway (105688); Butyrate Response Factor 1 (BRF1) Destabilizes MRNA Pathway (187213); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811)

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.2 PublicationsManual assertion based on experiment in:Ref.13

48-Strip-Wells

565美元

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150