human toll-like receptor 2; TLR2 ELISA Kit; CD282; TIL4; toll/interleukin 1 receptor-like 4; toll-like receptor 2
toll-like receptor 2; Toll-like receptor 2; toll-like receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4; toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; CD_antigen: CD282
This assay has high sensitivity and excellent specificity for detection of Human TLR2. No significant cross-reactivity or interference between Human TLR2 and analogues was observed.
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078pg/ml
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Immunity
For Samples For the quantitative determination of human human human human human toll oll oll -like ike ike ike receptor 2 eceptor 2 eceptor 2 eceptor 2 eceptor 2 eceptor 2 eceptor 2 (TLR2) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TLR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI GI登录号 :
Activated TLR4 Signalling Pathway (106400); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Beta Defensins Pathway (530759); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Defensins Pathway (530757); Hepatitis B Pathway (694606); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865)
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]
Function: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
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