ELISA/assay

人类基质金属蛋白酶12, 基质金属蛋白酶-12酶联免疫吸附试剂盒

MBS701937

48-Strip-Wells

565美元

酶联免疫吸附试剂盒

人类基质金属蛋白酶12, 基质金属蛋白酶-12酶联免疫吸附试剂盒

matrix metallopeptidase 12 (macrophage elastase)

Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit; HME; MGC138506; MME; macrophage elastase; macrophage metalloelastase; matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase) ; matrix metallopeptidase 12 (macrophage elastase)

macrophage metalloelastase preproprotein; Macrophage metalloelastase; macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase); matrix metallopeptidase 12 (macrophage elastase); Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12

MMP12

MMP12;MMP12;ME;HME;MME;基质金属蛋白酶-12;HME;MME;ME;hME;基质金属蛋白酶-12

MMP12_HUMAN

人类

470

This assay has high sensitivity and excellent specificity for detection of human MMP-12. No significant cross-reactivity or interference between human MMP-12 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human MMP-12 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

73858572

NP_002417.2

NM_002426.4

P39900

54,002 Da

Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Catalytic activity: Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala- -Leu-15 and 16-Tyr- -Leu-17 in the B chain of insulin. Cofactor: Binds 4 calcium ions per subunit.

48-Strip-Wells

565美元

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150